络通对不同病程糖尿病大鼠视网膜微血管细胞凋亡的影响

Effects of Rutosids on Apoptosis of Retinal Capillary in Rats with Different Courses of Diabetes

目的:探讨络通对不同病程糖尿病大鼠视网膜微血管细胞凋亡的影响。方法:采用链脲佐菌素65mg/kg经大鼠腹腔一次性注射制备糖尿病模型。成模糖尿病大鼠随机分成模型组、成模后即开始络通干预6个月组(络通干预组)和成模6个月后开始络通治疗3、6个月组(络通治疗1、2组)。分别按期摘取眼球,制备视网膜消化铺片微血管标本,PAS染色及核苷酸末端转移酶介导dUTP缺口翻译法(TUNEL法)特异性标记凋亡细胞,光镜下观察。结果:与同期模型组比较,络通干预组视网膜毛细血管周细胞凋亡现象不明显;络通治疗1组周细胞凋亡比较常见,少数内皮细胞出现凋亡;络通治疗2组治疗6个月内皮细胞凋亡较治疗3个月时有所增多,但明显轻于12个月模型对照组。结论:络通对糖尿病大鼠视网膜微血管病变早中期的周细胞凋亡有较好的抑制作用。

Objective： To research the effects of Rutosids on apoptosis of retinal capillary in rats with different courses of diabetes. Methods： Diabetes models were prepared by injecting streptozotocin 65mg/kg via rats＇ abdominal cavity. Diabetes rat models were randomly divided into four groups in terms of diabetes model group （DMG）, Rutosids intervention group （RIG）, Rutosids therapy group 1 （RTG1） and Rutosids therapy group 2 （RTG2）. Eyeballs were selected separately, retinals digesting stretched preparation capillary samples were prepared, and apoptosis cells were specificity marked by PAS dyeing and ribonucleotide terminal transierase mediating dUTP nick-translation （TUNEL method）, to observe below light microscope. Resuits： Compared with the homeechronous DMG, the apeptosis of retinal pericapillary cells in RIG was not obvious; perithelial cell apoptosis of RTG1 was relatively common, a few endothelial cells were apeptosis; endotheliocyte apoptosis of RTG2 was increased after treating for 3 months, but obviously lighted than 12 months model group. Conclusion： Rutosids has the prefer- able inhibitory action on perithelial cell apoptosis of early metaphase retinal microangiopathy in diabetes rots.