氨哮素雾化吸入对哮喘儿童血液淋巴细胞β肾上腺素能受体的影响

Influence of Inhalation of Clenbuterol on Beta-adrenoceptors of Lymphocytes in Asthmatic Children

目的：研究雾化吸入氨哮素对哮喘儿童血液淋巴细胞β受体的影响。方法：采用放射配基结合分析法和异丙基肾上腺素刺激实验。结果：哮喘儿童的β受体数目（Ｂｍａｘ）和功能（ｃＡＭＰ）及肺功能（ＰＥＦＲ）较健康儿童显著下降；雾化吸入氨哮素５ｄ后肺功能明显改善，但并不损伤β受体的数目和功能。结论：短期内雾化吸入治疗剂量的第３代β２受体激动剂不会出现低敏感现象。

To investigate the influence of inhalation of clenbuterol on beta-adrenoceptors of peripheral lymphocytes in asthmatic children. METHODS: The radioligand binding assays and ISO-stimulated assays were used. RESULTS: The density (Bmax) and the function (cAMP) of beta-adrenergic receptors in asthmatic children and their lung function (PEFR)were significantly decreased compared with those of healthy individuals. After inhalation of clenbuterol for 5 days,a significant improvement of lung function was shown,and damage of the density and the function of beta-adrenoceptors was not found. CONCLUSIONS: No subsensitization was found during a short-term inhalation of the third generation β2-agonist at a therapeutic dose.