早孕胎盘滋养层细胞的免疫组化研究

Immunocytochemical Demonstration of Trophoblasts in the Early Pregnancy

目的：研究早孕胎盘绒毛和子宫蜕膜中合体滋养层细胞、细胞滋养层细胞和中间滋养层细胞的免疫组化特征。方法：对２０例妊娠头３个月的刮宫标本进行Ｋｅｒａｆｉｎ、ｈＣＧ、ＨＰＬ、ＰＬＡＰ、ＥＭＡ和Ｐｒｏｌｅｃｔｉｎ等免疫组化染色。结果：角蛋白是各种滋养层细胞最可靠和最敏感的标记物，但其特异性差。合体滋养层细胞的ｈＣＧ和ＰＬＡＰ染色强于中间滋养层细胞，而中间滋养层细胞的ＨＰＬ和ＥＭＡ染色强于合体滋养层细胞。除角蛋白外，细胞滋养层细胞免疫组化染色均阴性。结论；早孕时三种滋养层细胞在免疫组化方面有其共性，也有各自不同的特点。

OBJECTIVES: To study immunocytochemical features of syncytiotrophoblast(ST), cytotrophoblast (CT) and intermediate trophoblast(IT)in the early pregnancy. METHODS: Twenty specimens of the first trimester villi and deciduas were stained with antibodies to cytokeratin, hCG, HPL, PLAP, EMA and Prolactin. RESULTS:Cytokeratin is the most sensitive and reliable marker for all types of trophoblasts though lacking specificity. Furthermore hCG and PLAP stained ST is stronger than IT, HPL and EMA stained iT stronger than ST. In contrast, CT showed negative stain for all immunocytochemical markers used in this study but cytokeratin. CONCLUSIONS: Though three trophoblasts in the early pregnancy share somewhat common feature's each trophoblast is of its own characteristics immunocytochemically.