SELDI技术检测反义寡核苷酸作用前后肝癌细胞培养液的差异蛋白

Detection of differential protein in culture fluid of hepatocellular carcinoma cells treated by antisense oligodeoxynucleotide using SELDI

目的利用针对人端粒酶RNA(hTR)的反义寡核苷酸(ASODN)和正义寡核苷酸(NODN)作用于人肝癌细胞SMMC-7721,比较ASODN和NODN作用后细胞培养液蛋白的变化。方法利用SELDI技术检测差异蛋白的表达变化。对照组为未加任何处理因素的SMMC-7721细胞。结果SELDI技术检测发现,ASODN作用组有40个差异蛋白分子低表达,3个差异蛋白分子高表达。NODN作用组有37个差异蛋白分子低表达,6个差异蛋白分子高表达。所有差异蛋白分子量均小于10 000 Da。ASODN作用组中有3个低表达蛋白在NODN作用组高表达,分子量分别为3 011.99 Da、3 048.28 Da和3 248.75 Da。结论ASODN和NODN作用SMMC-7721细胞后细胞培养液中所表达的差异蛋白十分相似。

Objective To compare the changes of protein expression in culture fluid of hepatocellular carcinoma SMMC-7721 cells treated by antisense oligodeoxynucleotide （ASODN） and nonsense oligodeoxynucleotide （NODN） aimed at human telomerase RNA. Methods SELDI technology was used to detect the changes of protein expression. The control group was hepatocellular carcinoma SMMC-7721 cells without any disposal. Results The results of SELDI showed that 40 proteins downregulated and 3 proteins up-regulated in ASODN group, 37 proteins down-regulated and 3 proteins up- regulated in NODN group. The molecular weight of these different proteins were all less than 10 000 Da. Three proteins （3 011.99 Da, 3 048.28 Da, 3 248.75 Da） down-regulated in ASODN group, but up-regulated in NODN group. Concluions The expression of different proteins in AODN group and in NODN group were extremely similar.