儿童外伤性白内障晶状体上皮细胞的线粒体观察

Morphology observation of the filament of the lens epithelial cells in the traumatic cataract in children

目的研究儿童外伤性白内障晶状体上皮细胞的线粒体形态变化。方法用日本产AEM-1200型透射电镜对10例儿童外伤性白内障及正常晶状体上皮细胞的线粒体进行观察。用计算机图像分析系统计算线粒体的体积密度。结果儿童外伤性白内障晶状体上皮细胞与正常对照组相比线粒体肿胀、变空非常明显,嵴变形扭曲且大量减少或消失,呈现空泡化,内膜和外膜均有破损。两组线粒体的体积密度差异有显著性(P<0.05)。结论儿童外伤性白内障晶状体上皮细胞的线粒体病理学改变与白内障的发生发展密切相关。

Objective To observe the morphology change of the filament of the lens epithelial cells in the traumatic cataract in children. Methods The filament of LECs of traumatic cataract （ 10 cases） in children and filament of normal lens were observed by AEM 1200 type electron microscope. The filament of LECs in the two groups were caculated with computer image analysis system. Results In the traumaticcataract, the filament of LECs swelled and emptied obviously, the cristaes were deformated and distorted, and significantly reduced, disappear and emptied. The inside membrane and the outside membrane of the filament were damaged. There was significant difference in Vv between the two groups （P〈 0.05）. Conclusions The morphology change of the filament of the lens epithelial cells may play a critic role in pathogenesis of the traumatic cataract in children.