HPLC法测定毛果芸香碱色谱条件的筛选

election of HPLC condition of assaying pilocarpineQian Chunmei, Lin ET and Tse MM

探讨了利用正相硅胶－反相洗脱系统测定毛果芸香碱的ＨＰＬＣ色谱条件。结果表明，流动相（乙腈为主体）中的含水量、磷酸与三乙胺的用量、ｐＨ都程度不同地改变该药物的保留时间。最佳色谱条件是：检测波长２１７ｎｍ，流动相为含０．００５％磷酸的８０％乙腈（以１ｍｏｌ／ＬＮａＯＨ调节ｐＨ＝５）。

The HPLC conditions ware studied for assaying pilocarpine by normalsilica column--reverse elution system. The retention time of piloarpine changed with theproportion of water to acetonitrile,amount of phosphoric acid and triethylamine and pHvalue of the mobile phase. At last the best chromatographic conditions had set. The detective wavelength is 217nm. The mobihe phase is 80% acetonitrile containing 0. 005% phosphoric acid and adjusted pH to 5 with 1mol/L sodium hydroxide.