链脲佐菌素诱导大鼠糖尿病肾病模型的建立

Establishment of a streptozotocin-induced rat model of diabetic nephropathy

目的:建立大鼠糖尿病模型,探讨其肾脏损害规律。方法:用链脲佐菌素(STZ)56mg/kg一次性腹腔内注射方式制作糖尿病大鼠模型,设正常对照组,饲养10周,期间观察大鼠血糖及一般情况变化,实验结束时测定血浆葡萄糖、血胰岛素、糖化血红蛋白、血尿素氮、24h尿量、24h尿蛋白、尿白蛋白,取肾作病理检查。结果:模型组大鼠葡萄糖、糖化血红蛋白、血尿素氮、24h尿量、24h尿蛋白、尿白蛋白明显升高,血胰岛素显著下降;病理显示出现肾脏肥大,明显肾小球、肾小管病变。结论:STZ诱导糖尿病大鼠出现肾脏功能受损,表现为肾小球及肾小管间质损害,可作为糖尿病肾病动物模型用于糖尿病肾病防治研究。

Objective To establish a rat model of diabetic nephropathy and to study its kidney impairment. Methods Forty male Spradely rats were randomly divided into control and experimental group （20 for each）. 20 rats in the experiment group were intraperitoneaUy injected with streptozotocin （STZ） at a dose of 56 mg/kg to develop an animal model of diabetes. All of the rats were fed for 10 weeks and their blood glucose was measured and gross healthy status was observed during this period of time, At the end of this experiment, their plasm glucose, fast insulin, Hemoglobin Ale （HbAle）, blood urea nitrogen （BUN）, 24-hour urine volume, 24-hour urine protein and urine albumin were assayed. A pathologleal examination was performed on the kidney by light microscopy. Results It showed a significant increase on blood glucose, HbAle, BUN, 24-hour urine volume, 24-hour urine protein and urine albumin, and decrease on fast insulin in this rat model. And the pathological examination exhibited kidney hypertrophy with impairment of glomerulus and renal tubule. Conclusion The STZ-induced rat model of diabetes developed pathological changes in glomerulus and interstitium and this animal model could be used to study human diabetic nephropathy.