银杏叶提取物对慢性阻塞性肺疾病患者诱导痰炎症细胞计数及IL-8水平的影响

Effects of Ginkgo biloba extract(GBE)on the inflammatory cell and the level of interlukin-8 in induced sputum from patients with chronic obstructive pulmonary disease

目的:观察银杏叶提取物(GBE)对慢性阻塞性肺疾病(COPD)患者诱导痰IL-8的影响,研究GBE治疗COPD的疗效,并初步探讨其作用机制。方法:36例COPD缓解期患者随机分为GBE治疗组和安慰剂治疗组,分别于治疗前后行诱导痰炎性细胞计数、ELISA法测诱导痰上清中IL-8含量以及肺功能检查。并与18例健康人比较。结果:COPD患者治疗前后各指标与健康人比较差异均有显著性(P<0.01);GBE治疗组治疗后诱导痰中性粒细胞百分比(PMN%)、IL-8水平均显著性下降(P<0.05),FEV1占预计值百分比显著性升高(P<0.05)。而安慰剂组治疗前后各指标差异无显著性(P>0.05)。结论:GBE可减轻COPD患者气道炎症反应,改善肺功能而发挥治疗作用。

Objective To explore, the effect and mechanism of Ginkgo biloba extract （GBE） on interlukin-8 （IL-8） in induced sputum from the patients with chronic obstructive pulmonary disease （COPD） and evaluate GBE in the treatment of COPD. Methods . Thirty six patients with COPD in stable phase were randomly divided into two groups, the GBE treated group and the placebo group. The total count of the inflammatory cells and the pulmonary ventilatory function were measured and the level of IL-8 in the supernate of induced sputum was detected by ELISA before and after treatment. All of the data were compared with those from 18 healthy subjects. Results All the indexes measured in patients before and after treatment were significantly different from those in the healthy subjects （ P〈0.01 ）. The percentage of polymorpbonuclear neutrophil （PMN%） and the level of IL-8 in induced sputum in the GBE treated group significantly decreased after treatment （ P〈0.05）, while the percentage of forced expiratory volume in one second in its predicted value （FEV1/Pre%） and the ratio of FEV1/FVC （forced vital capacity） significantly increased （ P〈0.05）. But all of the indexes from the placebo group had no significantly changes （ P〉0.05）. Conclusion GBE could play a therapeutic role on patients with COPD due to improving the pulmonary ventilation by reducing the airway inflammatory reaction.