大豆对SMV SC-7株系群的抗性遗传与基因定位

Inheritance and Gene Mapping of Resistance to SMV Strain SC-7 in Soybean

科丰1号×南农1138-2的P1、P2、F1和180个重组自交家系接种SC-7株系群的鉴定表明,P1与F1全抗,P2全感,说明抗性为显性;重组自交家系抗、感按1∶1分离,说明抗性由一对基因控制。利用王永军等的遗传连锁图对SC-7株系群的抗性基因进行连锁分析,将抗病基因Rsc-7定位于N8-D1b+W连锁群上,并与已定位的5个抗性基因中的3个连锁,还有一个与之相连锁的标记LC5T,其排列顺序和遗传距离为Rsa(30.6 cM)Rsc-7(22.1 cM)Rn3(10.3 cM)Rn1(15.8 cM)LC5T。

The P1 . P2 . F1 and 180 RILs of Kefeng 1 × Nannong1138-2 were inoculated with the SMV strain SC-7 for identification of their resistance. The results showed Kefeng 1 being resistant, Nannong 1138-2 being susceptible, F1 being resistant, and the RILs segregating in a 1：1 ratio, which indicated that a dominant gene controlled the resistance to SC-7. With linkage analysis, the resistance gene Rsc-7 was located on the linkage group N8-D1b＋ W and linked with three of five resistance genes and a RFLP maker LCST. The order and genetic distance of linked genes were Rsa （30.6 cM） Rsc-7 （22.1 cM） Rn3 （10.3 cM） Rn1 （15.8 cM） LCST.