航天搭载与非搭载舟形藻(Navicula tenera)培养基的优化

Optimization of culture media for spaceflight and nonspaceflight Navicula tenera

通过单因子和正交实验探讨了培养液中不同浓度KNO3、Na2HPO4、FeC l3和Na2S iO3对通过“神舟5号”飞船搭载与非搭载舟形藻(Navicula tenera)生长的影响,确立了适合搭载与非搭载舟形藻生长的最佳培养基组合。结果表明,经搭载后舟形藻生长所需的营养盐组合发生了变化。通过正交实验筛选出适合搭载舟形藻生长的最佳培养基为含300 mg.L-1KNO3、60 mg.L-1Na2HPO4.12H2O、24 mg.L-1FeC l3和700 mg.L-1Na2S iO3.9H2O的F/2培养基;适合非搭载舟形藻生长的最佳培养基为含600 mg.L-1KNO3、60 mg.L-1Na2HPO4.12H2O、12mg.L-1FeC l3和700 mg.L-1Na2S iO3.9H2O的F/2培养基。

The optimal combinations of different concentrations of KNO3, Na2HPO4, FeCl3 and Na2SiO3 in culture media for spaceflight and nonspaceflight Navicula tenera were studied by single factor experiment and orthogonal experiment [ L16 （4^5） ]. The results indicated that the combinations and concentrations of nutrients in culture medium for N. tenera growth were changed after spaceflight. The best medium for growth of spaceflight N. tenera was F/2 containing 300 mg · L^-1 KNO3, 60 mg · L^-1 Na2HPO4 · 12H2O, 24 mg · L^-1 FeCl3 and 700 mg · L^-1 Na2SiO3 · 9H2O, while the best medium for nonspaceflight N. tenera was F/2 containing 600 mg · L^-1 KNO3, 60 mg · L^-1 Na2HPO4 · 12H2O, 12 mg · L^-1 FeCl3 and 700 mg · L^-1 Na2SiO3·9H2O.