血管紧张素原基因核心启动子(AGCEl)G-6A变异与新疆哈萨克族原发性高血压的相关性研究

The association between the G-6A allele of angiotensinogen gene core promoter element l(AGCEl)

目的研究血管紧张素原(AGT)基因核心启动子(AGCEl)部位G-6A分子变异与中国新疆哈萨克族原发性高血压的关系。方法应用聚合酶链反应(PCR)结合限制性酶切(RFLP)方法对85名新疆哈萨克族血压正常者与85例新疆哈萨克族原发性高血压(EH)患者进行G-6A分子突变的检测。结果AGT基因G-6A多态性各基因型在EH组和对照组中的分布分别为8.25%、37.64%、54.11%和32.94%、34.12%、32.94%,差异有统计学意义(χ2=17.126,P<0.001),-6A等位基因在EH组(72.94%)的分布高于对照组(50.00%),差异有统计学意义(χ2=18.888,P<0.001),携带A等位基因EH的患病风险度是携带G等位基因的2.696倍(OR=2.696,95%CI:1.714～4.239);女性EH患者与对照组的差别更大。结论AGT基因AA基因型可能与新疆哈萨克族EH的发生有关,-6A等位基因是EH发病的危险因子,并且与性别表达有关,对新疆哈萨克族女性的影响更大。

Objective To investigate whether the variants G-6A of angiotensinogen gene are involved in the pathogenesis of essential hypertension in Kazakans. Methods This case-control study recruited 85 subjects from Kazakans of Xinjiang. PCR combined with restrict enzyme digestion was used to detect the variants. Results The distribution frequency of G-6A allele had 2.696 fold increased risk for hypertension. The relationship was significant in females but not in males. Conclusion The allele of G-6A may be associated with pathogenesis of essential hypertension in Xinjiang Kazakans and has more influence on females.