血小板第4因子对辐射损伤小鼠免疫功能的保护作用

Protective effects of platelet factor 4(PF4) on the immune function of the radiation injury mice

目的:探讨血小板第4因子(p latelet factor 4,PF4)对辐射损伤小鼠免疫系统的影响。方法:雄性BALB/c小鼠随机分为3组,第Ⅰ组(单纯照射组)、第Ⅱ组(PF4保护组)、第Ⅲ组(正常对照组),第Ⅱ组在照射前26 h及20 h腹腔注射PF4 40μg/kg,全身一次性5 Gy60Co-γ射线照射后瑞氏染色法计数外周血淋巴细胞百分数。胸腺、脾脏制成细胞悬液计数后,MTT比色法测定胸腺及脾细胞增殖能力。结果:第Ⅱ组小鼠外周血淋巴细胞百分数下降趋势较第Ⅰ组明显减慢。胸腺、脾细胞计数,第Ⅱ组明显高于第Ⅰ组。淋巴细胞增殖试验,第Ⅱ组与第Ⅰ组相比,小鼠胸腺及脾细胞的增殖能力明显增强。结论:PF4对免疫系统有辐射保护作用,可明显增强辐射损伤小鼠的免疫功能。

Objective.To study the effects of platelet factor 4 （PF4） on the immune function of the radiation injury mice. Methods： BALB/c male mice were randomly divided into three groups： exposure group, protection group with PF4 and control group. The mice of protection group were injected PF4 （40 μg/kg） into abdominal cavity at 26 h and 20 h before irradiation of 5 Gy^60Co -γ ray. The percentage of lymphocytes in peripheral blood was counted by Wright＇s staining. After counting the total cells of thymus and spleen, the cellular proliferation ability of thymus and spleen was determined by MTI＇ colorimetry. Results： The falling trendency of the peripheral blood lymphocytes in protection group was obviously slower than that in exposure group. The total cells of thymus and spleen in protection group was much more than that in exposure group. Comparing the cellular proliferation ability of thymus and spleen between protection group and exposure group, the former was better than the latter. Conclusion： PF4 has obvious protective effects on the immune system and can strengthen the immune function of the radiation injury mice.