紫外线照射充氧自血回输对兔急性梭曼中毒血清一氧化氮及一氧化氮合酶水平的影响

Effects of ultraviolet blood irradiation and oxygenation on the levels of nitric oxide and nitric oxide synthase in rabbits with acute soman intoxication

目的:探讨紫外线照射充氧自血回输(UB IO)治疗梭曼急性中毒的机理。方法:建立兔急性梭曼中毒模型。100只家兔随机分为正常对照组、中毒组、常规治疗组、UB IO治疗组及UB IO加常规治疗(复合治疗)组。观察14 d,检测兔血清一氧化氮(NO)及一氧化氮合酶(NOS)水平的变化。结果:正常对照组中兔血清NO及NOS水平分别为(8.38±3.40)μmol/L及(19.25±2.11)U/m l,中毒组中兔血清NO及NOS水平分别为(6.81±1.57)μmol/L及(17.46±1.12)U/m l,两组间有显著性差异(P<0.05)。经UB IO和复合治疗后,NO浓度分别为(7.88±2.05)及(8.03±2.46)μmol/L,NOS水平分别为(19.23±2.67)及(18.73±2.51)U/m l,与中毒组比较有显著差异(P<0.05)。结论:梭曼急性中毒后可使血清NO及NOS水平发生改变,此改变可能参与梭曼中毒的损伤,UB IO治疗对NO、NOS有极为有益的调节作用,能有效地治疗梭曼急性中毒。

Objective： To study the therapeutic mechanism of ultraviolet blood irradiation and oxygenation（UBIO） for acute soman intoxication in rabbits. Methods ： The rabbit model of acute soman intoxication was established. One hundred rabbits were randomly divided into five groups, including normal control,intoxication, routine therapy, UBIO therapy and UBIO combined with routine therapy （ complex therapy）. After fourteen - day observation , levels of serum nitric oxide（NO） and nitric oxide synthase（NOS） were determined. Re,stilts ：The levels of NO and NOS in normal control group were （ 8. 38 ± 3.40 ） μmol/L and （ 19.25 ± 2. 11 ） U/ml respectively, while those in intoxication group were（6.81±1.57）μmol/L and （17.46 ±1.12）U/ml, and the difference was significant（P 〈 0.05）. After UBIO and complex therapy , the levels of NO were （ 7.88 ±2.05 ） μmol/L and （8.03±2.46） μmol/L respectively, and the levels of NOS were （ 19.23± 2.67 ）U/ml and （ 18.73± 2.51 ）U/ml respectively. There was a significant difference compared with intoxication group （ P 〈 0.05 ）. Conclusion： Acute soman intoxication may affect the levels of serum NO and NOS activity. This change may be related to the injury by soman intoxication. UBIO therapy can regulate the NO and NOS activities and is effective for acute soman intoxication.