转座子报告基因Tn5gusA5诱变甘蓝黑腐病菌分离与致病相关的突变体

Mutagenesis of Xanthomonas campestris pv. campestris with Transposon Tn5gusA5 and Isolation of Mutants Impaired with Pathogenicity

构建携带报告基因的转座子Ｔｎ５ｇｕｓＡ５于广谱性ｃｏｓ质粒ｐＬＡＦＲ１上，利用Ｔｎ５ｇｕｓＡ５诱变野生型甘蓝黑腐病黄单胞菌，筛选与致病性有关的因子（胞外多糖，胞外酶）突变体，通过Ｓｏｕｔｈｅｒｎ杂交和报告基因ｇｕｓ表达验证，突变体是由转座子诱变所致。

A pLAFRl:: Tn5gusA5 transposon has been constructed that fuse the Escherichia coli gusA reporter gene on cosmid pLAFRl, it was used to mutagenize wild type of Xanthomonas campestris pv. campestris which causes black rot of crucifer. Mutants impaired pathogenicity was isolated and proved it being caused by Tn5gusA5 inactivation through evidence of Southern analysis and gus reporter expression.