干扰素-γ加强肿瘤坏死因子相关凋亡诱导配体抑制神经母细胞瘤细胞的作用

IFN-γ enhance the ability of the inhibition of TRAIL to neuroblastoma cell line

目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人神经母细胞瘤的作用及干扰素-γ(IFN-γ)对新型凋亡分子TRAIL抗瘤活性的影响,并探讨其影响机制。方法应用MTT分析和流式细胞仪研究TRAIL对人神经母细胞瘤细胞的抑制作用及IFN-γ对TRAIL抗瘤活性的影响。用RT-PCR方法检测IFN-γ作用48h后,Caspase-8-mRNA的表达。结果TRAIL(10,50,100μg/L)单独作用、IFN-γ(1000U/mL)单独作用后,对SY5Y细胞的增殖抑制率分别为(4·38±0·89)%、(3·54±1·66)%、(5·03±1·26)%、(5·33±2·06)%;凋亡率分別为(5·18±3·33)%、(5·26±3·64)%、(5·00±2·88)%、(8·14±7·35)%。IFN-γ(1000U/mL)与TRAIL(100μg/L)联合作用后,对SY5Y细胞的增殖抑制率为(41·22±7·09)%;凋亡率为(21·29±6·20)%。RT-PCR方法发现IFN-γ作用48h后Caspase-8-mRNA的表达明显上调。结论神经母细胞瘤细胞SH-SY5Y对TRAIL不敏感,IFN-γ可以明显提高TRAIL对神经母细胞瘤细胞的敏感性,其发生机制可能是通过IFN-γ上调Caspase-8-mRNA的表达而实现的。

Objective To explore the effect of TRAIL on neuroblastoma cell line and the influence of IFN-γ on the antitumor effects of TRAIL. Methods Use MTT and flow cytometric analysis to detect the antitumor effects of TRAIL and study the effects of IFN-γ on TRAIL in inhibiting human neuroblastoma cells. Results The rates of killing of TRAIL alone,IFN-γ alone and the combination of TRAIL and IFN-γ were （ 5.03 ± 1.26 ） %, （ 5. 33 ± 2. 06 ） %, （ 41.22 ± 7.09 ） %. IFN-γ could enhance the killing rate of TRAIL to the SYSY cells. FCM analysis suggested that IFN-γ enhanced the killing rate of TRAIL to the SYSY cells based on inhibiting apoptosis of SYSY cells. Conclusion SH-SYSY cells are not sensitive to TRAIL. IFN-γ can upregulate the apoptosis of SYSY induced by TRAIL.