芦竹碱法合成DL-色氨酸

The Synthesis of DL-tryptophan with Gramine

以芦竹碱和海因为原料,经缩合、水解合成DL-色氨酸,收率达41.6%。缩合反应的最佳工艺条件为:反应时间5 h、碘甲烷与芦竹碱的摩尔比为1.2∶1、乙醇钠与芦竹碱的摩尔比为0.8∶1、原料芦竹碱与海因的摩尔比为0.9∶1;水解反应的最佳工艺条件为:NaOH浓度为1.5 mol/L、水解时间10 h、吲哚甲基海因的浓度为0.2 mol/L、温度为100℃。

DL-tryptophan was synthesizd by condensing gramine with hydantoin and subsequent hydrolyzation of 5-（3-indole-methyl）hydantoin intermediate. The yield of DL-tryptophan to gramine was up to 41.6%. The optimum conditions of condensation were： 10 h reacdon time, 1.2：0. 8：1 molar ratio of CH3I to C2HsONa to gramine, 0. 9：1 molar ratio of gramine to hydantoin. The optimum conditions of hydrolysis were ： 1.5 mol/L NaOH, 10 h hydrolysis time, 0. 2 mol/L 5-（3-indolemethyl）hydantoin, and 100 ℃ reaction temperature.