连续性神经瘤型损伤模型兔的CNTF和CGRP表达变化的研究

Research about the expression changes of CNTF and CGRP in the rabbits' model of neuroma-in-continuity

目的通过造成兔腓神经部分损伤,形成连续性神经瘤损伤的哺乳类动物模型,为进一步研究提供基础。方法随机选取12只雄性新西兰兔一侧腓神经的部分束被切除,6周后损伤段神经组织切片经苏木精伊红染色、Luxol fast blue组化染色证实形成典型神经瘤病理改变；以建立损伤模型的6只兔子健侧作为对照,用实时荧光定量PCR法测定损伤神经远端以及L_7、S_1背根神经节睫状节神经营养因子(CNTF)、降钙素基因相关肽(CGRP)的mRNA表达量变化,以Western-blot法测定CNTF、CGRP的蛋白表达水平。结果腓神经形成连续性神经瘤后神经瘤侧CNTF、CGRP的mRNA和蛋白表达与健侧对照比较,其变化为CNTF的mRNA和蛋白表达量下降(P＜0．05),而CGRP的mRNA和蛋白表达量上调(P＜0．05)。结论腓神经的部分损伤方法可有效地建立连续性神经瘤型损伤的模型,并引起CNTF、CGRP的表达变化。

Objective In view of being short of the mammalian model in neuroma-in-continuity, the experiment injured the part of peroneal nerve to the formation of the neuroma-in-continuity and was applied to the foundation of farther research. Methods Twelve New Zeland rabbits were selected as experimental subjects randomly. One lateral peroneal nerves of twelve rabbits were resected, the damaged nervous tissues＇ slice were showed to the typical pathological changes of neuroma by the stain of HE, luxol fast blue after six weeks. As compared with the health sides of six model rabbits, the methods of real-time PCR and Western blot were used to evaluate the expression of CNTF, CGRP mRNA and protein in injured nerves and L7 ,S1 dorsal root ganglions respectively. Results The injured nerve formed the typical pathological changes of neuroma at six weeks. Compared with healthg side the expression of CNTF mRNA and protein was down-graded at the lateral of neuroma（ P 〈 0. 05 ）, and the expression of CGRP mRNA and protein was up-graded （ P 〈 0. 05 ）. Conclusion The method of partly injuring the peroneal nerve could effectively set up the model of the neuroma-incontinuity, furthermore, resulted to the expression changes of the CNTF, CGRP mRNA and protein.