夏枯草及抗炎一号注射液经肝动脉联合灌注治疗大鼠肝癌作用机制的初步探讨

Mechanism of prunella vulgaris L and Kangyan No.1 infused via hepatic artery against primary liver cancer of rat

目的:探讨夏枯草及抗炎一号注射液经肝动脉联合灌注治疗大鼠肝癌的作用机制。方法:建立30只二乙基亚硝胺(DEN)诱发大鼠晚期原发性肝癌模型,随机分为中药组、化疗组和盐水组,每组各10只。经胃十二指肠动脉至肝固有动脉分别灌注生理盐水0.5ml、夏枯草注射液0.6g/kg+抗炎一号注射液0.45g/kg、顺铂(DDP)4mg/kg+羟基喜树碱(HCPT)1.5mg/kg+5-氟尿嘧啶(5-FU)34mg/kg。应用流式细胞仪(FCM)检测治疗后各组大鼠肝癌细胞的凋亡情况。结果:盐水组、化疗组和中药组均存在不同程度的细胞凋亡现象,与盐水组比较,中药组和化疗组肝癌细胞凋亡百分率均显著增高(P<0.01),但化疗组和中药组之间无显著性差异(P>0.05)。结论:夏枯草与抗炎一号注射液可诱导大鼠肝癌细胞凋亡,其程度与化疗药物相似。

Objective： To study the mechanism of prunella vulgaris L and Kangyan No. 1 infused via hepatic artery against rat primary liver cancer. Methods： Thirty rats with advanced primary liver cancer induced by DEN were di vided into 3 groups randomly, each group 10 rats and were infused with normal saline, prunellae vulgaris L and Kan gyan No. I, and chemotherapy（ include DDP ＋ HCPT ＋ 5 - FU） from gastroduodenal artery into hepatic artery re spectively. Hepatocellular carcinoma cell apoptosis was detected by flow cytometry（FCM） in each group after treatment. Results： Each group had apoptosis phenomenon in some degree. Compared with NS group, TCM group and chemotherapy groupg apoptosis index were higher significantly（ P 〈0.01 ）, but had no statistics difference between TCM group and ehemiotherapy group（P 〉0.05）. Conclusion： Prunellae vulgaris 1 and Kangyan No. I could induce apoptosis of rat hepatoceUular carcinoma cells as well as chemiotherapy drug.