Effects of ischemic preconditioning on cyclinD1 expression during early ischemic reperfusion in rats

瞄准：观察效果是在老鼠肝细胞的 cyclinD1 表示上的化学家 preconditioning 在期间早是化学家灌注。方法：54 只 SD 老鼠随机被划分成是 preconditioning 组(IP ) ， ischemia/reperfusion 组(红外) 和假冒的操作组织的化学家(那么) 。IP 和红外组进一步被划分成四亚群(n = 6 ) 。假冒的操作组(那么) 担任了控制组(n = 6 ) 。部分肝 ischemia/reperfusion 的一个模型被使用，在哪个老鼠在灌注以前为 60 min 受到肝局部缺血。在 IP 组的动物经历了在 ischemia/reperfusion 挑战以前每次为 5 min 是化学家 preconditioning 两次。在灌注的 0， 1， 2，和 4 h 以后，在每个组的浆液和肝织物被收集检测浆液中高音，肝组织病理学说和 cyclinD1 mRNA 和蛋白质的表示的水平。流动血细胞计数被用来作为房间新生的数量指示物检测房间周期。结果：与红外组相比， IP 组在 1 h 显示出显著地更低的中高音水平到 4 h 亚群(P 【 0.05 ) 。增长索引(PI ) 由 S 阶段和 G2/M-phase 比率显示了[(S+G2/M )/(G0/G1+S+G2/M )] 显著地在 0 和 1 h 在 IP 组被增加(26.44 +/-7.60% 对 18.56 +/-6.40% ， 41.87 +/-7.27% 对 20.25 +/-6.70% ， P 【 0.05 ) 。同时， cyclinD1 蛋白质表示能在 IP 组被检测。但是在红外组，， cyclinD1 蛋白质表示发生了在灌注以后的 2 h。在 IP 显著地增加的 cyclinD1 mRNA 的表示在 0 和 1 h 组织(0.568 +/- 0.112 对 0.274 +/- 0.069， 0.762 +/- 0.164 对 0.348 +/- 0.093， P 【 0.05 ) 。结论：局部缺血 preconditioning 能保护肝细胞免于 ischemia/reperfusion 损害，它可能早与房间增长和 cyclinD1 的表示有关在期间是化学家灌注。

AIM： To observe the effect of ischemic preconditioning on cyclinD1 expression in rat liver cells during early ischemic reperfusion. METHODS： Fifty-four SD rats were randomly divided into ischemic preconditioning group （IP）, ischemia/ reperfusion group （IR） and sham operation group （SO）. The IP and IR groups were further divided into four sub-groups （n = 6）. Sham operation group （SO） served as the control group （n = 6）. A model of partial liver ischemia/reperfusion was used, in which rats were subjected to liver ischemia for 60 min prior to reperfusion. The animals in the IP group underwent ischemic preconditioning twice for 5 min each time prior to the ischemia/reperfusion challenge. Alter 0, 1, 2, and 4 h of reperfusion, serum and liver tissue in each group were collected to detect the level of serum ALT, liver histopathology and expression of cyclinD1 mRNA and protein. Flow cytometry was used to detect cell cycle as the quantity indicator of cell regeneration. RESULTS： Compared with IR group, IP group showed a significantly lower ALT level in 1h to 4h sub-groups （P 〈 0.05）. Proliferation index（PI） indicated by the S-phase and G2/M-phase ratio [（S＋G2/M）/（G0/G1＋S＋G2/M）] was significantly increased in IP group at 0 and 1 h （26.44 ± 7.60% vs 18.56 ± 6.40%,41.87 ± 7.27% vs 20.25 ± 6.70%, P 〈 0.05）. Meanwhile, cyclinD1 protein expression could be detected in IP group. But in IR group, cyclinD1 protein expression occurred 2 h alter reperfusion. The expression of cyclinD1 mRNA increased significantly in IP group at 0 and 1h （0.568 ± 0.112 vs 0.274 ± 0.069, 0.762 ± 0.164 vs 0.348 ± 0.093,P 〈 0.05）. CONCLUSION： Ischemic preconditioning can protect liver cells against ischemia/reperfusion injury, which may be related to cell proliferation and expression of cyclinD1 during early ischemic reperfusion.