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应用SELDI-TOF-MS技术分析SEB染毒小鼠血清蛋白质组学变化 被引量：7

Application of SELDI-TOF-MS technology in detection of serum proteomic changes of mice exposed to staphylococcal enterotoxin B
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摘要 目的:把蛋白质芯片和SELDI质谱技术应用于金黄色葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)染毒小鼠血清蛋白质组研究,分析染毒小鼠血清蛋白质组的变化．方法:采用表面增强激光解吸电离飞行时间质谱技术和弱阳离子交换蛋白质芯片检测染毒小鼠血清蛋白质的变化,使用PBSⅡ-C 型蛋白质芯片阅读机读取数据,获得的结果采用Ciphergen公司的Biomarker wizard和 Biomarker Patterns System软件进行分析．结果:实验组与对照组血清蛋白质谱相比有 11个蛋白质有显著差异,其中8个蛋白质表达上调,3个表达下调．结论:SEB染毒小鼠血清蛋白质组发生显著变化,可能与SEB的毒理学与病理学作用有密切关系． AIM： To study the changes of serum proteome in mice exposed to staphylococcal enterotoxin B （SEB）. METHODS： The serum proteins in mice exposed to SEB were detected by surfaced enhanced laser desorption/ionization time of flight mass spectroscopy （SELDI-TOF-MS） and weak cation exchange proteinchip. The data were read with PBSII-C type proteinchip reader and analyzed with Biomarker Wizard and Biomarker Patterns Software of Ciphergen Company. RESULTS： in comparison with the control group, there were significant deviations of 11 proteins in the test group. Among them, 8 proteins were upregulated and 3 down-regulated. CONCLUSION： Marked serum proteomic changes exist in mice exposed to SEB, which may be related to the toxicity and pathological effect of SEB.
作者 张靖 张军 应天翼 高川 宋云扬 王惠芳
机构地区 防化研究院第四研究所
出处 《世界华人消化杂志》 CAS 北大核心 2006年第11期1111-1114,共4页 World Chinese Journal of Digestology
关键词 SELDI-TOF-MS蛋白质芯片 SEB 标志蛋白 SELDI-TOF-MS Protein biochip Staphylococcal enterotoxin B Marker protein
分类号 R115 [医药卫生—公共卫生与预防医学]
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参考文献16

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二级参考文献24

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共引文献32

同被引文献106

引证文献7

二级引证文献13

世界华人消化杂志
2006年 第11期

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