中药酒速愈对急性酒精中毒小鼠肝脏氧化酶的影响

Effect of traditional Chinese medicine Jiusuyu(酒速愈) on oxygenase in liver tissue of mice with acute alcoholism

目的:观察中药酒速愈对急性酒精中毒小鼠肝组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(M DA)含量的影响。方法:采用乙醇体积分数为56%的红星二锅头灌胃建立急性酒精中毒小鼠模型。80只健康昆明种小鼠按随机数字表法分为正常对照组、模型组、酒速愈高剂量组、酒速愈低剂量组和葛花解酲汤组,每组16只。各组于给药后6 h分别检测小鼠肝组织SOD、CAT活性和M DA含量。结果:与正常对照组比较,模型组小鼠肝组织M DA含量明显升高(P<0.01),SOD和CAT活性均明显降低(P均<0.01);与模型组比较,各治疗组均可升高小鼠肝组织SOD和CAT活性(P<0.05或P<0.01),并可降低M DA含量(P<0.05或P<0.01);与葛花解酲汤组比较,酒速愈高、低剂量组小鼠肝组织CAT活性均明显升高(P均<0.05)。结论:酒速愈能显著升高模型小鼠肝组织SOD和CAT活性,降低M DA含量,抑制脂质过氧化反应,具有抗氧化作用,从而通过调节和改善自由基代谢,减轻急性酒精中毒对肝脏的氧化损伤。

Objective： To observe the effect of traditional Chinese medicine Jiusuyu （酒速愈） on activities of superoxide dismutase （SOD） and catalase （CAT）, as well as the content of malondialdehyde （MDA） in liver tissues of mice with acute alcoholism. Methods： The models of acute alcoholism mice were established by drinking 56% Hongxing Erguotou （红星二锅头） drink. Eighty healthy Kunming mice were randomly divided into normal group, model group, high Jiusuyu dosage group, low Jiusuyiu dosage group and Gehua Jiecheng decoction （葛花解醒汤） group, with 16 animals in each group. The SOD, CAT activities and the content of MDA in liver tissues of each group were detected 6 hours after treatment. Results ： The content of MDA in liver tissues of the model group was increased （P〈0.01）, and the activities of SOD and CAT were decreased in liver tissues of the model group compared with those in the normal control group （all P〈0.01）. Compared with model group, the activities of SOD and CAT in the liver tissues of the treatment groups were increased （P〈0.01 or P〈0.05）, the content of MDA in liver tissues of each treatment group was decreased （P〈0.05 or P〈0.01）. Compared with Gehua Jiecheng decoction group, the activities of CAT in liver tissues of high Jiusuyu dosage and low Jiusuyu dosage groups were increased （both P〈0. 05）. Conclusion： Jiusuyu could improve the SOD and CAT activities and decrease the content of MDA in liver tissues and could repress the lipid peroxidation, so it could relieve the oxidative injury of liver tissues from acute alcoholism by regulating and improving the oxygen free radical metabolism.