制备真皮类似物促进表皮样结构形成

导出

摘要以表皮细胞直接进行立体培养,尽管可以观察到表皮细胞的生长及分层,但是无典型的表皮结构,究其原因,可能与缺乏诱导表皮生长发育的微环境有关,真皮结构,尤其是成纤维细胞及其基质对表皮的正常分化和功能具有重要作用.我们利用真皮类似物,结合空气-液面培养技术,模拟体内环境,使完整的表皮结构得以形成.

作者耿松梅雷小兵曾维惠谭升顺彭振辉肖生祥

机构地区西安交通大学第二医院皮肤科

出处《中华皮肤科杂志》 CAS CSCD 北大核心 2006年第6期362-363,共2页 Chinese Journal of Dermatology

关键词真皮类似物表皮样构形制备表皮结构表皮细胞生长发育成纤维细胞立体培养培养技术

参考文献7

1Lamme EN, Van Leeuwen RT, Brandsma K, et al. Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation. J Pathol,2000, 190: 595-603.
2Black AF, Bouez C, Perrier E, et al. Optimization and characterization of an engineered human skin equivalent. Tissue Eng, 2005, 11:723-733.
3Lee DY, Ahn HT, Cho KH. A new skin equivalent model: dermal substrate that combines de-epidermized dermis with fibroblast-populated collagen matrix. J Dermatol Sci, 2000, 23: 132-137.
4侯伟,Surinder Mangal,孙建方,Cynthia Lehe,Mieke Bergers,Pranab.K.Das.体外重建皮肤与正常人皮肤基本特征的对比研究[J].中华皮肤科杂志,2006,39(2):61-63. 被引量：1
5Pinney E, Liu K, Sheeman B, et al. Human three-dimensional fibroblast cultures express angiogenic activity. J Cell Physiol, 2000,183: 74-82.
6Yang EK, Seo YK, Youn HH, et al. Tissue engineered artificial skin composed of dermis and epidermis. Artif Organs, 2000, 24: 7-17.
7Wha Kim S, Lee IW, Cho HJ, et al. Fibroblasts and ascorbate regulate epidermalization in reconstructed human epidermis. J Dermatol Sci, 2002, 30: 215-223.

1Rheinwald JG,Green H.Serial cultivation of strains of human epidermal keratinocytes:the formation of keratinizing colonies from single cells.Cell,1975,6:331-343.
2Olsson MJ,Moellmann G,Lerner AB,et al.Vitiligo:repigmentation with cultured melanocytes after cryostorage.Acta Derm Venereol,1994,74:226-228.
3Regnier M,Patwardhan A,Scheynius A,et al.Reconstructed human epidermis composed of keratinocytes,melanocytes and langerhans cells.Med Biol Eng Comput,1998,36:821-824.
4Burgeson RE,Christiano AM.The dermal-epidermal junction.Curr Opin Cell Biol,1997,9:651-658.
5Haake A,Scott GA,Holbrook KA.Structuture and function of the skin.In:Freinkel RK,Woodley DT.The biology of the skin.New York:Pathenon Publishing Group,2001.19-45.

1王廷明,汪岩锋,陈晓白,林定州,王丰明.腹壁术后切口处表皮样囊肿3例报告[J].腹部外科,1997,10(1).
2魏民,刘玉杰,李众利,王志刚,朱娟丽.关节镜清理结合灌注冲洗治疗前交叉韧带重建术后关节感染[J].中国骨伤,2015,28(3):279-281. 被引量：7
3陈志方,谢文扬,宋琦,李萍,邓飞.CD44v6在粘液表皮样癌中的表达[J].遵义医学院学报,2003,26(1):22-23.
4黎炽彬,王安训.口腔颌面部皮样或表皮样囊肿20例临床分析[J].新医学,1996,27(12):646-646.
5王强,邱武安,张红星.手指末节指骨内表皮样骨囊肿一例[J].中华手外科杂志,2013,29(2):78-78. 被引量：2
6常英展,罗伟,缪东生,姜凤娥,王旭平,何飞.鼻腔及鼻窦黏液表皮样癌30例临床分析[J].中国耳鼻咽喉头颈外科,2006,13(10):683-684. 被引量：2
7张宝燕,邹仲之,朴英杰.含人发角蛋白的真皮类似物植入大鼠皮下的形态学变化[J].解剖学杂志,2003,26(4):327-330.
8王钢,李亚明.小切口结合围手术期综合康复训练治疗创伤性膝关节僵直27例[J].中国中医骨伤科杂志,2010,18(12):57-58.
9董建平,罗志伟,董辉.老年大脑半球内巨大表皮样囊肿一例[J].实用肿瘤杂志,1995,10(4):239-239.
10李春智,孙沫逸,张晓晖,王贺忠,尉文华.涎腺粘液表皮样癌术后转移的临床与病理研究[J].口腔颌面外科杂志,2004,14(4):342-344. 被引量：5

中华皮肤科杂志

2006年第6期

内容加载中请稍等...