雌激素受体在人舌癌细胞系中的表达

Expression of estrogen receptor in human tongue cancer cells

目的:测定雌激素受体(ER)在人舌癌细胞系Tca8113以及高转移株Tb上的表达。方法:用免疫细胞化学方法和RT-PCR方法检测雌激素受体在人舌癌细胞上的表达,并对其表达量进行相对定量分析。结果:经免疫细胞化学和RT-PCR方法可检测到ER在人舌癌细胞上有表达。ERα在Tb细胞系的表达强于Tca8113;而ERβ的表达在Tca8113和Tb中相同,并且ERα/ERβ比值在脑转移株Tb中升高。结论:雌激素有可能通过它的受体对人舌鳞癌细胞产生一定的影响。ERα/ERβ比值的改变在舌癌发生和转移过程中可能起一定作用。

Objective： To observe the expression of estrogen receptor alpha （ERα） and beta （ERβ） in human tongue cancer cell line Tca8113 and its highly metastatic cell line Tb. netheds： Immunocytochemistry, RT-PCR methods were used to observe the expression of ERs in the in vitro cultured human tongue cancer Tca8113 and Tb cells. Results：ERa and ERβ mRNA were expressed in both cell lines, and the expression of ERα in Tb cells was stronger than that in Tca8113 cells, the expression of ERβ was the same in the two cell lines. The ratio of ERα to ERβ was higher in Tb cells than that in Tca8113 cells. Conclusion.The overexpression of ERα and ERα/ERβ ratio may be associated with the higher potential of metastasis of Tb cells.