浙江汉族人群KIR3DL2等位基因的多态性分析

Allelic polymorphism analysis of killer cell immunoglobulin-like receptor gene 3DL2 in Zhejiang Han population

目的探讨浙江汉族人群KIR3DL2等位基因的多态性分布情况。方法KIR3DL2等位基因分型采用聚合酶链反应寡核苷酸探针杂交方法,利用引物特异性扩增KIR3DL2基因的两个片段,合成21条探针检测KIR3DL2基因的多态性位点,根据探针的格局判断出样本的KIR3DL2等位基因情况。结果样本中共发现17种探针格局,检测到7种等位基因,其中KIR3DL2002等位基因占57%。1例样本无法用现有等位基因探针格局进行分析。结论聚合酶链反应寡核苷酸探针杂交方法可有效检测KIR3DL2等位基因,浙江汉族人群KIR3DL2等位基因的分布有其特点。

Objective To analyze the allelic polymorphism of killer cell immunoglobulin （Ig）-like receptor （KIR） gene 3DL2 in Zhejiang Han population. Methods AUelic definition within KIR3DL2 was achieved through using the PCR-sequence specific oligonucleotide probes （PCR-SSOP） method and the specific amplification of exon 3-exon 4 and exon 8-exon 9. Twenty-one digoxigenin-labelled probes were used to the SSOP analysis. Results Seven alleles of KIR2DL2 were observed, in which KIR2DL2 ＊ 002 had the highest allele frequency, 0.57. One sample could not be given the allele combination according to the probe profile. Conclusion The PCR-SSOP method was reliable. There is a distinctive allele frequency of KIR2DL2 in Zhejiang Han population.