Decorin mRNA和p57^(KIP2) mRNA在非小细胞肺癌中的表达及其临床意义

Study on expression of Decorin mRNA and p57^(KIP2) mRNA and their clinical significance in non-small cell lung cancer

目的检测Decorin mRNA和p57^(KIP2) mRNA在非小细胞肺癌中的表达情况,并分析它们与非小细胞肺癌的病理、临床特征及预后的关系。方法选取石蜡包埋的59例非小细胞肺癌及12例癌旁正常肺组织的标本制作组织芯片。利用原位杂交法检测Decorin mRNA和p57^(KIP2) mRNA的表达情况。结果Decorin mRNA在癌旁正常肺组织和非小细胞肺癌组织中的表达率分别为66.7%(8/12)和20.3%(12/59),正常组织中Decorin mRNA的表达高于肺癌组织(P<0.05)。大细胞肺癌中Decorin mRNA的表达高于鳞癌(P<0.05)。鳞癌中p57^(KIP2) mRNA的阳性表达率为63.2%(12/19),高于腺癌、大细胞癌和小细胞癌(P<0.05)。在无淋巴结转移的非小细胞肺癌标本中Decorin mRNA和p57^(KIP2) mRNA的表达呈增高趋势,与有淋巴结转移的标本比较其差异具有统计学意义。Decorin mRNA和p57^(KIP2) mRNA的表达无相关性(P>0.05)。结论Decorin和p57^(KIP2)的表达可能与非小细胞肺癌的组织学类型和转移有关,有望成为监测非小细胞肺癌患者病情发展及评价预后的有用指标。

Objective：To investigate the expression of Decorin mRNA and p57^KIP2 mRNA and analyze their relationship with pathogenesis and clinical characteristics in non-small cell lung cancer. Methods：Paraffin-embedded tissues from 59 cases of non-small cell lung cancer and 12 cases of normal lung tissues adjacent to tumor tissues were selected to make tissue chip, and expression of Decorin mRNA and p57^KIP2 mRNA were examined by in situ hybridization using labelled digoxigenin probes. Results：The expression rates of Decorin mRNA were 20. 3% （12/59） in tumor tissues and 66, 7% （8/12） in normal tissues, The normal tissues showed a higher expression of Decorin mRNA than that of the tumor tissues （ P 〈 0. 05 ）. The expression rate of Decorin mRNA was higher in large cell lung adenocarcinoma（LCLC） than that of squamous cell carcinoma of the lung（ LSCC）, The positive expression rates were 5.3% （ 1/19）, 50% （4/4） respectively（P 〈0. 05）. The expression rate of p57^KIP2 mRNA in LSCC was 63. 2% （ 12/19）, being higher than adenocarcinoma of the lung（LAC）, LCLC and alveolar carcinoma （P 〈0. 05）. In non-small cell lung cancer without lymph node metastasis, The expression of Decorin mRNA and p57^KIP2 mRNA was increased significantly（ P 〈 0. 05 ）. However there was no correlation between Decorin mRNA and p57^KIP2 mRNA. Conclusion： These results suggest that Decorin gene and p57^KIP2 gene closely relate to the non-small cell lung cancer metastasis and histological classification. The two genes may be an index in monitoring the status and evaluating the prognosis of the non-small cell lung cancer.