转基因抗虫棉SSR和AFLP遗传变异研究

Genetic Variation of Transgenic Insect-resistant Cottons Using SSR and AFLP Markers

利用SSR和AFLP两种分子标记技术,分析了52份转基因抗虫棉品种(系)的遗传多样性。结果表明:在61对SSR引物中,有4对引物在供试材料中表现出多态性,共扩增出102个标记,其中多态性标记25个,多态性百分率为24.51%,每对引物的扩增带数变化在17～30之间;在100对AFLP引物中,有9对引物在供试材料中产生多态性,共扩增出618个标记,多态性标记33个,占总数的5.34%,每对引物组合扩增的标记数分布于47～81之间。成对品种的欧式距离变化在2.00～5.57之间,平均值为4.21,单一品种欧氏距离的平均值分布在3.73～4.75之间,表明不同品种之间遗传差异不大。基于SSRs和AFLPs多态性数据的聚类分析,可以将供试材料划分为3个类群(SAGs),但类群划分与品种地理来源不十分吻合。

In the paper, genetic variation of 52 transgenic insect-resistant cottons was analyzed using SSR and AFLP molecular markers. The results showed among 61 SSR primers 4 could produce polymorphic markers. A total of 102 DNA fragments was scored, among which 25 （24.51%） bands were polymorphic. The number of DNA bands per primer ranged from 17 to 30. Nine AFLP primer combinations were selected to perform AFLP fingerprinting from 100. The total bands and polymorphic bands were 618 and 33 （accounted for 5.34%）, respectively. The number of DNA bands per primer combination ranged from 47 to 81. Mean pairwise genetic distance of 52 varieties （lines） was 4.21, with the range of 2.00 to 5.57. Mean genetic distance for each variety ranged from 3.73 to 4.75. The limited genetic variation was revealed by these results in the population. Based on dendrogram of SSRs and AFLPs, 52 varieties （lines） were divided into three SSR/AFLP-based groups （SAGs）. However, SAGs was not accordance with geographic origins of tested varieties （lines）.