晋旱125×昔野501杂交后代抗旱生理指标的研究

Selective Indexes of Drought-resistance for Hybrid Descendents of Jinhan125×Xiye501

以晋旱125×昔野501杂交组合的亲本及选育的有代表性的4个特殊类型的后代为试验材料,通过实验室检测和大田考察相结合的方法,在正常供水和干旱胁迫的条件下,测定苗期和花荚期与抗旱性相关的生理指标,在花荚期水分敏感期对叶片相对含水量、持水力、脯氨酸含量、超氧化物歧化酶、过氧化物酶以及酯酶同工酶等酶活性及谱带进行测定和分析,初步认为这一群体中抗旱性从强到弱的次序为:晋旱125、731(尖叶白花)、732(圆叶紫花)、昔野501、733(圆叶白花)、734(尖叶紫花)。

Four lines including Jinhan125, Xiye501 and their cross offspring were selected as test materials. Physiological and ecological indexes relevant to drought-tolerance in seedling and flower-podding stage were determined by laboratory testing and field observation. In the condition of normal water supply and drought stress, the flower-podding stage was the most sensitive stage to water content in whole stages. We determined relative water content,ability of water holding, proline content, activity of POD, SOD, EST and analyzed their bands. The result indicated that the order of drought-tolerance was as follows： Jinhan125, 73-1, 73-2, Xie501, 73-3, 73-4.