中青年脑梗死患者血管紧张素原基因M235T多态性的研究

The study of angiotensinogen gene M235T polymorphism with cerebral infarction in young and middle-aged adults.

目的探讨血管紧张素原(AGT)基因M235T多态性与中青年脑梗死(C I)的关系。方法应用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)对62例脑梗死患者,58例正常人进行AGT基因M235T多态性分析。结果脑梗死组AGT基因T235等位基因频率为79.84%,235TT基因型频率为64.52%,对照组分别为73.28%和55.17%。两者的差异无统计学意义χ2=1.09,P=0.296;χ2=1.443,P=0.230,235MT、235MM基因型频率两组相比也无差异(P>0.05)。结论AGT基因M235T多态性可能与中国山西汉族中青年人群C I发病无关。

Objective： To explore the relationship between angiotensinogen （AGT） gene M235T polymorphism and cerebral infarction in young and middle - aged adults. Methods ： M235T polymorphism of AGT gene from peripheral blood leukocytes was examined in 62 cases with CI and 58 noninfarct controls by polymerase chain reaction （PCR） amplification and restriction fragment length polymorphism （RFLP）. Results ： The frequencies of AGT gene T235 allele and 235TT genetype were 79. 84% and 64. 52% respectively which are little higher than those of the control group （73.28% and 55.17% respectively）. There is no statistical difference be- tween them （χ^2= 1.09, P = 0. 296 ; χ^2 = 1. 443, P = 0, 230 ）. Conclusions ： There might be no association between AGT gene M235T and cerebral infarction in young and middle -aged adults.