临产前后胎盘组织bcl-2、Fasl的表达与母血、羊水EGF变化的关系

A relationship between the expressions of bcl-2 and Fasl in placental and the concentrations of EGF in maternal blood and amniotic fluid on labor onset.

目的探讨临产前后bc l-2、Fasl在胎盘滋养层细胞的蛋白表达变化及其与母血、羊水中表皮生长因子(EGF)的浓度之间的关系。方法53例足月妊娠妇女分成两组,其中临产组28例,未临产组25例。用免疫组化法测定胎盘滋养层细胞bc l-2、Fasl的蛋白表达,用放射免疫法测定孕妇母血、羊水中EGF的浓度。结果(1)临产组bc l-2、Fasl的表达明显低于未临产组(P<0.05)。(2)临产组母血、羊水EGF水平高于未临产组(P<0.05)。(3)母血、羊水中EGF的变化与bc l-2的表达呈负相关,而与Fasl的表达不相关。结论bc l-2、Fasl介导的胎盘滋养层细胞凋亡在启动分娩发动过程中具有重要作用;EGF可能对bc l-2的表达存在下调作用。

Objective： To investigate the expressions of bcl - 2 and Fasl proteins in placental trophoblastic cells on labor onset and their relationships with the concentrations of Epidermol Growth Factors （ EGF） in maternal blood and amniotic fluid. Methods： Fifty - three women on term pregnancy were divided into two groups. One group on the time of labor onset were twenty - eight women. Another group not on the time of labor onset were twenty - five women. Expressions of bcl - 2 and Fasl proteins in placental trophoblastic cells were detected by immunohistochemical assay. The concentrations of EGF in maternal blood and amniotic fluid were detected by radioimmune assay. Results ： （ 1 ） Expressions of bcl - 2 and Fasl in labor onset group were significantly lower than those of the group not labor onset（ P 〈0. 05）. （2） The concentrations of EGF in maternal blood and amniotic fluid in labor onset group were higher than those of not labor onset （ P 〈 0. 05 ）. （3） The concentrations of EGF in maternal blood and amnionotic fluid had a negative correlation with the expression of bcl - 2, but had not correlation with Fasl, Conclusions ： It was considered that the apoptosis of placental trophoblastic cells induced by bcl - 2 and Fasl may play an important role in the initiation of labor onset. And EGF may had a down - regulation to the expression of bcl -2 .