草珊瑚药材中依斯坦布林A的HPLC法测定

Determination of Istanbulin A in Sarcandra glabra

目的:建立了HPLC法对草珊瑚中依斯坦布林A的含量测定方法。方法:采用RP-HPLC外标法,色谱柱为hypersil ODS18柱(4．6 mm×200 mm,5μm),以甲醇-水(40:60)溶液为流动相,检测波长218 nm。结果:依斯坦布林A在0．135-2．16μg范围内线性关系良好,平均回收率为98．54％。结论:该方法快速简捷,精密度高,重现性好,可用于草珊瑚中依斯坦布林A含量的测定。

Objective： To determine istanbulin A in Sarcandra glabra （Thunb.） Nakai. Methods： An RP-HPLC method was established and an hypersil ODS C18 column （4. 6 mm×200 mm,5μm） was used. The detective wavelength was at 218nm. Results： Istanbulin A had a good linearity in the range of 0. 135～2. 16μg. The recovery rate was 98. 54%. Conclusion： This method is simple, accurate and repeatable. It can be used to determine istanbulin A in Sarcandra glabra.