摘要
目的:建立了HPLC法对草珊瑚中依斯坦布林A的含量测定方法。方法:采用RP-HPLC外标法,色谱柱为hypersil ODS18柱(4.6 mm×200 mm,5μm),以甲醇-水(40:60)溶液为流动相,检测波长218 nm。结果:依斯坦布林A在0.135-2.16μg范围内线性关系良好,平均回收率为98.54%。结论:该方法快速简捷,精密度高,重现性好,可用于草珊瑚中依斯坦布林A含量的测定。
Objective: To determine istanbulin A in Sarcandra glabra (Thunb.) Nakai. Methods: An RP-HPLC method was established and an hypersil ODS C18 column (4. 6 mm×200 mm,5μm) was used. The detective wavelength was at 218nm. Results: Istanbulin A had a good linearity in the range of 0. 135~2. 16μg. The recovery rate was 98. 54%. Conclusion: This method is simple, accurate and repeatable. It can be used to determine istanbulin A in Sarcandra glabra.
出处
《中药材》
CAS
CSCD
北大核心
2006年第5期443-444,共2页
Journal of Chinese Medicinal Materials
基金
江西省自然科学基金(No10140015)
江西教育厅重点资助项目