桃儿七中主要生物活性成分的萃取技术研究

Study on Extraction Methods of Activity Components from Podophyllum emodi Wall.

采用超临界CO3流体萃取技术及常规溶剂萃取方法对桃儿七的主要化学成分进行萃取和分离,并用高效液相色谱法测定其成分,得出用超临界CO2萃取桃儿七根茎中木脂素类的鬼臼和脱氧鬼臼及黄酮醇类的山奈酚和槲皮素的较适工艺条件。对试验结果分析讨论后认为:超临界CO2萃取桃儿七中木脂素类优于常规溶剂提取方法,并对超临界CO2流体萃取桃儿七工业化应用前景和可行性进行了分析探讨。

Supercritical fluid Extraction （SFE） with subsequent HPLC analysis was utilized to extract activity components such as podophyllotoxin, deoxypodophyllotoxin, kaempferol and quercetin from the roots and stems of Podophyllum emodi Wall. The extraction yields obtained by SFE were compared to those obtained by organic solvent extraction and were found to be comparable or lower. In addition, we used the orthogonal experiment which conducts with 3 factors and 4 levers in order to find the best dynamic extraction conditions.