半夏泻心汤对肝郁脾虚大鼠生长抑素和胃动素的影响

Effects of "Banxia Xiexin Doction" on Somatostatin and Motilin in Rats of Liver Depression and Spleen Deficiency

目的探讨半夏泻心汤对肝郁脾虚动物中枢和外周组织中胃肠激素生长抑素(SS)、胃动素(MTL)的含量及其与胃肠运动的关系。方法采用大黄灌胃加应激束缚方法诱导大鼠,造成肝郁脾虚模型,然后将动物随机分为半夏泻心汤不同剂量组、模型组、西药组(西沙比利)、空白对照组,分别接受相应药物治疗。应用放射免疫法和免疫组化法,观察大鼠中枢和外周各组织中胃肠激素生长抑素(SS)、胃动素(MTL)的含量,并对脑肠肽含量进行定量、定性的测定及半夏泻心汤对其影响。结果肝郁脾虚模型动物出现中枢和外周两方面的胃肠激素紊乱,外周MTL减少,SS增高,中枢则MTL、SS均有增加。半夏泻心汤各剂量组则对该紊乱表现出不同的调节作用,小剂量组能上调外周MTL而中枢作用不明显;中剂量组能降低外周SS含量和分布,尤以胃、十二指肠为主,中枢作用亦不明显;大剂量组则着重于中枢调节,使中枢MTL、SS均增加,以MTL增加较明显。结论半夏泻心汤对胃肠激素的调节作用,依据其剂量大小,其作用的部位有所侧重,表现在从外周到中枢的调节变化,以及促进胃肠运动和抑制胃肠运动的逐渐转变。半夏泻心汤对偏亢或偏抑状态下的胃肠运动的双向调节作用可能与其对胃肠激素的这种外周、中枢的不同调节作用有关。

Objective To observe the effects of ＂Banxia Xiexin Decoction＂ adjusting motilin and somatostatin and gastric movement. Methods The number and gray level of the positive SS cells and MTL cells were determined by immunohistochemistry method and radioimmunity method in different tissues.Results The positive SS cells determined in blood plasma, gastric antrum, duodenum,colon,especially high level in blood plasma and gastric antrum. The positive MTL cells determined in hypothalamus, the number and gray level of the cells significantly affected （P〈： 0.05）.Conclusion The results indicate that ＂Bamda Xiexin Decooction＂ can adjust SS and MTL in different tissues, and it can keep balance of gastric movement.