贯叶连翘总提取物对11株致病细菌的抗菌作用

Research into Antibacterial Effects of the Total Extract of Hypericum perforatum L. on 11 Strains of Clinically Isolated Gram-positive Bacteria

目的:探讨贯叶连翘总提取物对临床分离11株对常用抗生素有耐药性的革兰氏阳性细菌的抗菌作用.方法:用平板抑菌法、最低抑菌浓度(MIC)检测法、最低杀菌浓度(MBC)检测法检测了提取物抗菌作用的强弱,并以其中1株菌为代表检测了其在12 h培养过程中提取物作用时的光密度(OD640 nm)值和活菌数(CFU/mL).结果:提取物对所有菌株均有较强的抑菌和杀菌作用.结论:该结果提示提取物可用于控制临床上对多种抗生素有耐药性的革兰氏阳性菌株所致的感染.

Objective： The antibacterial effects of the total extract of Hypericum perforatum L. on 11 strains of clinically isolated antibiotic-resisting gram-positive bacteria were investigated. Method： Inhibition zones, minimum inhibitory concentra- tions （MIC） and minimum bactericidal concentrations （MBC） were tested. Besides, optical density at 640 nm （OD640.m） and colony forming units （CFU） of one of the strains were tested in the course of 12 hours＇ incubation. Result： The total extract had strong inhibitory and bactericidal effects on all of the strains. Conclusion： The total extract of Hypericum perforatum L, might be used in getting rid of infections caused by antibiotic-resisting gram-positive bacteria.