p,p’-DDE对离体培养支持细胞DNA损伤与FasL基因表达的影响

Effect of p,p'-DDE on DNA damage and expression of FasL gene of rat sertoli cell in vitro

目的研究p,p’-DDE对离体培养支持细胞DNA损伤与FasL基因表达的影响。方法从大鼠睾丸组织中分离支持细胞进行离体原代培养3天,加入不同浓度p,p’-DDE继续培养24h,应用单细胞凝胶电泳(SCGE)和反转录聚合链式反应(RT-PCR)研究p,p’-DDE诱导支持细胞DNA损伤和FasL基因表达。结果发现支持细胞DNA迁移度随着p,p’-DDE剂量的增高而增高,同时FasL的基因表达水平也随之增高。结论p,p’-DDE可诱导支持细胞DNA损伤和FasL基因表达,pp’-DDE可能是通过Fas/FasL途径诱导支持细胞损伤,破坏生精过程的动态平衡,最终导致精子减少。

Objective To study the effects of p, p＇-DDE on DNA damage and expression of FasL gene of rat sertoli cell in vitro. Methods After separating sertoli cells from testicular tissue of rats, we cultured these cells for 3 days and then added different doses of p, p＇-DDE into the culture medium to study its effect on DNA damage and FasL gene expression with the method of SCGE and RT-PCR. Results DNA migration and FasL gene expression of Sertoli cell significantly increased with the increase of the dose of p, p ＇-DDE. Conclusion p, p＇-DDE could induce DNA damage and FasL gene expression of sertoli cells of rat testis, p, p＇-DDE might induce the sertoli cell damage and destroy the dynamic process of sperolatogenesis through the Fas/FasL pathway, which might lead to oligozoospermatism.