共轭亚油酸对胰岛素抵抗大鼠ap2基因表达的影响

Effect of conjugated linoleic acid on the gene expression of the fatty acid binding protiens in resistant rat

目的通过研究共轭亚油酸(CLA)对饮食诱导胰岛素抵抗大鼠脂肪酸连接蛋白(ap2)基因表达的影响,探讨CLA抗糖尿病作用的机制。方法选用雄性Wistar大鼠,随机分为对照组、高脂组、高脂+CLA组(每100g饲料含CLA分别为0·75g、1·50g、3·00g),每组动物10只,观察CLA对胰岛素抵抗大鼠胰岛素、血糖水平的影响,并应用RT-PCR的方法检测ap2、过氧化物酶体增殖物活性受体γ(PPARγ)的表达水平。结果高脂组大鼠血清游离脂肪酸(FFA)、胰岛素和糖血水平显著高于基础组,CLA可降低胰岛素抵抗大鼠血清FFA、血糖、胰岛素水平,并可增加其脂肪组织ap2、PPARγmRNA的表达水平。结论CLA可通过激活PPARγ上调脂肪酸连接蛋白基因的表达,改善肥胖大鼠的胰岛素抵抗。

Objective To study the effect of conjugated linoleic acid on gene expression of fatty acid binding proteins （ap2）in white obesity tissue of rats with insulin resistance, and explore the mechanism of resisting diabetes by CLA. Methods Male Wistar rats were randomly separated to control group, high-fat group and high fat ＋ CLA group（0.75g, 1.50g,3.00g by diet weight）, Using reverse transcription polymerse chain reaction （RT-PCR） technique to measure the expression level of ap2 and Pernxisome prolifcrators-activated receptor - γ （PPARγ） mRNA expression, Results The FFA, serum insulin and glucose levels of rats with insulin resistance were higher than that of rats fed with basic diet, supplement of CLA can decrease FFA, serum insulin and glucose levels of rats with insulin resistance, and CLA can increase the expression of ap2 and PPARγ mRNA in adipose tissue of rat with insulin resistance. Conclusion CLA can improve insulin resistance of obese rat and increase the expression of ap2 mRNA, possibly acting through activing PPARγ.