葡萄皮提取物抗痴呆作用的实验研究

Anti-dementia effects of grape peel extracts experiment study

目的研究葡萄皮提取物抗痴呆效应及其机制。方法健康Wistar雄性大鼠,用D-半乳糖和亚硝酸钠腹腔注射建立拟痴呆模型,在造模同时分别给予生理盐水、脑复康、葡萄皮提取物低、中、高剂量干预。采用神经行为学及抗氧化类、胆碱能类和一氧化氮合酶、脑组织病理形态学和免疫组化等检测指标来进行观察、评价。结果葡萄皮提取物可明显改善拟痴呆模型大鼠的学习记忆功能,并可提高大鼠脑组织中超氧化物歧化酶、一氧化氮合酶、胆碱乙酰转移酶的活性,抑制丙二醛活性,减少模型组海马与皮质β-APP、β-AP阳性神经元细胞表达。结论葡萄皮提取物可抑制氧自由基形成,改善胆碱能神经系统的功能,增强大脑海马与皮层中一氧化氮合酶的活性,减少β-AP沉积斑块的形成,可能对早老性痴呆具有一定的防治作用。

Objective To study anti-dementia effects and mechanism of grape peel extracts on imitative dementia rats modle. Methods The imitative dementia rats modle were established using health male Wistar rats by intraperitoneal injection of D-galactose and NaNO2 , simultaneity, administer physiological saline solution, Piracetam Tablets, low dose grape peel extracts, middle dose grape peel extracts, high dose grape peel extracts to intervene separately. Using neurobehavioral tests, antioxidation genus, cholinesterase genus and nitric oxide synthase, histopathological and morphological parameters and immunohistochemical methods etc measure index to observe and evaluate. Results Grape peel extracts can significantly ameliorate learning and memory function of imitative dementia rats modle, and improve SOD enzyme, NOS enzyme, CAT enzyme activity in rats brain, inhibite MDA enzyme, AchE enzyme activity, reduce the expression of β-APP-positive immunoreactive neurocytes （β-APP ＋ ） and β-amyloid-positive immunoreactive neurocytes （β- AP ＋ ） in cerebra cortex and Hippocampus. Conclusion grape peel extracts can inhibite oxygen free radicel to form, amcliorate cholinomimetic neural function, enhance NOS enzyme activity in the hippocampus and cerebra cortex, reduce the development of β-AP deposited macula, grape peel extracts probability have a preventive action against early-aged dementia.