人肝组织病变及热凝固导致组织光学特性的变化

Pathological Changes and Thermal Coagulation of Human Liver Tissue Induced Changes in the Optical Properties of Liver Tissue at KTP/YAG Laser in vitro

研究了人肝的病变及热凝固的肝组织对532 nm和1064 nm激光的光学特性的变化。实验采用双积分球测量系统以及运用生物组织的反向倍增(IAD)光学模型。结果表明,正常肝组织对532 nm和1064 nm的吸收系数显著地较肝肿瘤组织要大;正常肝和肝肿瘤组织热凝固后对532 nm的吸收系数都显著地增大,正常肝组织热凝固后对1064 nm的吸收系数显著减小。而肝肿瘤组织热凝固后对1064 nm的吸收系数却显著地增大。正常肝组织对532 nm和1064 nm的散射系数都显著地较肝肿瘤组织要小,正常肝和肝肿瘤组织热凝固后散射系数都显著地增大。正常肝组织对532 nm和1064 nm的各向异性因子都明显地较肝肿瘤组织要大,正常肝和肝肿瘤组织热凝固后其各向异性因子都明显地减小。

Pathological changes and thermal coagulation of human liver tissue induced changes of the optical properties of liver tissue at 532 and 1064 nm wavelengths of laser in vitro. The measurements were performed using a double integrating-sphere setup, and the optical parameters were assessed from these measurements using the inverse adding-doubling method （IAD）. The results of measurement showed that the absorption coefficients of normal liver tissues at 532 and 1064 nm are significantly bigger than those of liver tumors at the same wavelength respectively. When liver tissues were coagulated by heat, the absorption coefficients of normal and tumorous liver tissues of thermal coagulation at 532 nm significantly increase, the absorption coefficient of normal liver tissues of thermal coagulation at 1064 nm significantly decreases, and the absorption coefficient of liver tumors of thermal coagulation at 1064 nm significantly increases. The scattering coefficients of normal liver tissue at 532 and 1064 nm are significantly smaller than those of liver tumors at the same wavelength. When liver tissues were coagulated by heat, the scattering coefficients of normal and tumorous liver tissue of thermal coagulation significantly increase. The anisotropy factors of normal liver tissue at 532 and 1064 nm are obviously bigger than those of liver tumors at the same wavelength respectively. When liver tissues were coagulated by heat, the anisotropy factors of normal and tumorous liver tissue of thermal coagulation at 532 and 1064 nm obviously decrease.