HPLC法测定糖泰胶囊中黄芩苷的含量

Determination of Baicalein in Tangtai Capsule by HPLC

目的采用高效液相色谱法测定糖泰胶囊中黄芩苷的含量。方法色谱柱:ZorbaxC18柱;流动相:乙腈-0.5%磷酸,梯度洗脱,1～15min乙腈含量15%～60%;流速:1.0mL/min;柱温:25℃;检测波长:280nm。结果线性范围为0.936～18.720μg,回归方程为:Y=4849.53X-0.9593,r=0.9999;平均回收率为98.29%,RSD=0.97%(n=5)。结论本方法简便、准确、重现性好,可用于该制剂的质量控制。

Objective A HPLC method to determine the content of Baicalein in Tangtai capsule was established. Method The separation was performed by Zorbax C18 column with acetonitrile and 0.5% phosphorice acid. The elution program was 1- 15 min, 15%-60% acetonitrile. The flow rate was 1 mL/min and column temperature was 25 ℃. Content was detected at a wavelength of 280 nm. Result The linear range was 0.936-18.72 μg, regression： Y=4849.53X-0.9593 （r=0.9999）. The average recovery rate was 98.29% and RSD was 0.97% （n =5）. Conclusion The method is simple, accurate and reproductive, and can be used for determining Baicalein in Tangtai capsule.