外源基因导入鲤(Cyprinus carpio)受精卵最佳时期的研究

THE RESEARCH OF THE OPTIMUM PERIOD TRANSFERED EXOGENOUS GENES INTO COMMON CARPS ZYGOTE

本研究以鲤鱼受精卵为材料，通过显微注射技术，在受精卵的不同发育时期把鲤鱼ＭＴ启动子基因和大麻哈鱼ＧＨ基因导入受精卵中，按孵出的鱼苗数分别计算成活率，再通过斑点杂交和Ｓｏｕｔｈｅｒｎ Ｂｌｏｔ杂交检测整合情况，计算整合率，孵化率最高的为单细胞中期（９０．８３％），整合率最高的为单细胞后期和囊胚期（４０％）。综合研究结果外源基因导入鲤鱼受精卵的最佳时期应选在单细胞后期。

Microinjection is main gene transfer technique in constructing transgenic fish. The egg survival rate and the ratio of the exogenous gene integration in genomic DNA is different in different development period of fertilized egg. Hear, it was reported that the relation of the fertilized egg survival rate. The ratio of exogenous gene integration in genomic DNA to the microinjeclion time in different development period of zygote.