大鼠角膜酸烧伤对角膜内皮细胞损伤和修复的实验观察

Experimental study on rat corneal endothelial damage and repair after acid burn

目的研究大鼠角膜酸烧伤对内皮细胞的损伤和影响内皮修复的因素。方法建立轻、中、重度大鼠角膜酸烧伤模型,裂隙灯下动态观察角膜水肿、混浊及上皮愈合情况;各组于烧伤后不同时间点取角膜行内皮细胞台盼蓝-茜素红联合活细胞染色及HE染色,观察内皮细胞损伤、修复及白细胞浸润情况。结果轻、中度酸烧伤角膜内皮细胞未见损伤改变,角膜少量白细胞浸润,上皮愈合及水肿消退均较快;重度酸烧伤损伤区内皮细胞坏死、脱落,修复期损伤周边区内皮细胞变形、伸长,向创面移行,角膜大量白细胞浸润,角膜上皮愈合延迟,水肿消退慢。结论重度酸烧伤可直接造成角膜内皮细胞受损,继发性炎症反应参与了酸烧伤后角膜内皮细胞的损害;内皮细胞受损可使角膜溃疡及水肿迁延不愈。

Objective To study the effect of acid burn on rat corneal endothelial damage and its repair. Methods The model of mild, moderate and severe acid burn in cornea was established in 90 Long-Evans rats （n = 30 for each degree of acid burn） with 4 rats served as normal controls. Corneal edema, opacity and the healing of epithelium were observed with a slit lamp microscope. The corneal endothelium from half rats of each degree acid burn was stained with trypan blue and alizarin red, and that from the other half with hematoxylin and eosin （HE） immediately, 30 min, 1, 3, 7, 14, 21, 28 d after cautery. The endothelial damage and its repair were observed and the number of the leucocytes in cornea was calculated under light microscope. Results No damage was seen in the corneal endothelial cells of mild, moderate acid burn, only a few leucocytes could be seen in the cornea, and the healing of epithelium, the regression of edema were within 7 d. The endothelial cells necrosed and shedded in the wound area by severe acid burn. The endothelial cells in the margin were deformed, elongated and slided to the damaged area during repair, and a great number of leucocytes infiltrated, the healing of corneal epithelium was delayed, the regression of edema was up.to 28 d. Conclusion The corneal endothelial cells were damaged in severe acid burn. The secondary in the damage of corneal endothelial cells after severe acid burn. The damage. healing of corneal ulcer and edema.