人骨关节炎滑膜成纤维细胞差异蛋白质谱初步分析

Analyses of differentially expressed proteins of human synovial fibroblasts obtained from osteoarthritis

目的应用双向凝胶电泳-质谱法分析正常人及骨关节炎(osteoarthritis,OA)患者滑膜成纤维细胞蛋白质组表达差异,探讨差异蛋白在骨关节炎发病中的作用。方法采用固相pH梯度(immob ilized pH grad ient,IPG)双向凝胶电泳(two-d imensional gel electrophoresis,2-DE)分离正常人及骨关节炎患者滑膜成纤维细胞总蛋白质,银染显色,PDQuest2-DE软件分析图像,比较2种细胞蛋白质组表达差异,对差异蛋白质点用基质辅助激光解析电离飞行时间质谱进行鉴定。结果胶图差异分析显示36个表达水平存在明显差异的蛋白质,选取15个点进行质谱鉴定,鉴定出6个表达上调蛋白质。结论正常人及骨关节炎患者滑膜成纤维细胞表达蛋白质组存在差异。这些差异蛋白可能参与骨关节炎的发病。

Objective To understand the possible roles of some proteins expressed by human synovial fibroblasts in the pathogenesis of osteoarthritis by means of 2-DE and bioinformatics. Methods The difference of synovial fibroblasts protein expression between osteoarthritis patients and healthy controls was analyzed by 2- DE. The differential expression spots were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry （MALDI-TOF-MS）, followed by bioinformatics analysis. Results Gel-image analysis revealed that there were 36 differential protein spots. A total of 15 differential protein spots were identified by MALDI-TOF-MS, of which 6 spots were much more intensive in osteoarthritis patients than normal ones. Conclusion These differential proteins based on bioinformatics analysis might be involved in synovitis in osteoarthritis patients.