期刊文献+

Effects of augmenting the migratory ability of mouse BMDC on immunotherapy

Effects of augmenting the migratory ability of mouse BMDC on immunotherapy
下载PDF
导出
摘要 Objective: Being antigen-presenting cells, dendritic cells (DCs) transport captured antigen from peripheral tissues to T cell zone of lymph nodes via lymphatic vessels. This migration is essential for the presentation of antigen that leads to priming of effector T cell responses. In this study, we tried to promote the migratory ability of mouse bone marrow-derived dendritic cells (BMDCs) loaded with antigen of breast cancer, and its immunological effect in vivo. Methods: After being loaded with breast carcinoma antigen, BMDCs were cultured with medium containing PGE2, LTC4, or Bryo-1 respectively. Phenotypic changes, CCR7 expression, chemotaxis assay, mixed lymphocyte response, specific T lymphocyte cytotoxicity assay and anti-tumor immune efficacy of BMDCs were observed. Results : PGF2 and LTC4 promoted maturation, CCR7 expression and migratory ability of BMDCs compared with control group in vitro. In vivo PGE2 and LTC4 group vaccines were more efficient on suppressing growth of mouse breast cancer than other groups. However Bryo-1 only enhanced BMDCs maturation. Conclusion: Because the effect of specific CTL in vitro had no difference, we suggested that migration of dendritic cells to lymph nodes maybe answered for the better anti-tumor immunological response induced by PGE2 or LTC4 in vivo. Objective: Being antigen-presenting cells, dendritic cells (DCs) transport captured antigen from peripheral tissues to T cell zone of lymph nodes via lymphatic vessels. This migration is essential for the presentation of antigen that leads to priming of effector T cell responses. In this study, we tried to promote the migratory ability of mouse bone marrow-derived dendritic cells (BMDCs) loaded with antigen of breast cancer, and its immunological effect in vivo. Methods: After being loaded with breast carcinoma antigen, BMDCs were cultured with medium containing PGE2, LTC4, or Bryo-1 respectively. Phenotypic changes, CCR7 expression, chemotaxis assay, mixed lymphocyte response, specific T lymphocyte cytotoxicity assay and anti-tumor immune efficacy of BMDCs were observed. Results : PGF2 and LTC4 promoted maturation, CCR7 expression and migratory ability of BMDCs compared with control group in vitro. In vivo PGE2 and LTC4 group vaccines were more efficient on suppressing growth of mouse breast cancer than other groups. However Bryo-1 only enhanced BMDCs maturation. Conclusion: Because the effect of specific CTL in vitro had no difference, we suggested that migration of dendritic cells to lymph nodes maybe answered for the better anti-tumor immunological response induced by PGE2 or LTC4 in vivo.
出处 《Journal of Nanjing Medical University》 2006年第3期133-140,共8页 南京医科大学学报(英文版)
关键词 dendritic cells breast carcinoma CHEMOKINES immunological therapy dendritic cells breast carcinoma chemokines immunological therapy
  • 相关文献

参考文献2

  • 1Deborah K. Armstrong,John A. Blessing,Janet Rader,Joel I. Sorosky.A randomized phase II evaluation of bryostatin-1 (NSC #339555) in persistent or recurrent squamous cell carcinoma of the cervix: A Gynecologic Oncology Group Study[J].Investigational New Drugs.2003(4)
  • 2Shadan Ali,Olivia Aranha,Yiwei Li,George R. Pettit,Fazlul H. Sarkar,Philip Agop Philip.Sensitization of human breast cancer cells to gemcitabine by the protein kinase C modulator bryostatin 1[J].Cancer Chemotherapy and Pharmacology.2003(3)

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部