睾酮在雄兔体内的抗动脉粥样硬化作用

Testosterone's Anti-Atherosclerosis Effect in Male Rabbits

目的探讨睾酮在雄兔动脉粥样硬化模型中是否具有血管保护作用。方法雄性白兔,饲以高脂饮食且分组。安慰剂组:行去势术并给予安慰剂;低睾酮组:行去势术并予睾酮0.25mg/kg·d;高睾酮组:行去势术并予睾酮2.5mg/kg·d;假手术组。3个月后检测血中睾酮水平、血脂、纤溶酶原激活物抑制物活性、一氧化氮、内皮素、血管紧张素Ⅱ含量,计算主动脉斑块占内膜面积百分比。结果安慰剂组血清睾酮水平明显低于其它三组(2.56±0.28μg/L比7.67±0.61μg/L、40.16±0.93μg/L和7.79±0.61μg/L,P<0.01);一氧化氮含量安慰剂组明显低于其它三组(39.0±9.5μmol/L比62.8±8.9μmol/L、56.7±10.3μmol/L和51.5±9.3μmol/L,P<0.05或P<0.01);纤溶酶原激活物抑制物活性安慰剂组高于低睾酮组(567.3±53.4u/L比334.4±51.3u/L,P<0.01);内皮素含量安慰剂组高于低睾酮组(911.9±15.4ng/L比846.1±43.0ng/L,P<0.05);血管紧张素Ⅱ含量安慰剂组高于低睾酮组(687.2±36.5ng/L比624.5±54.5ng/L,P<0.05);主动脉斑块占内膜面积百分比安慰剂组高于其它三组(46.4%±9.3%比20.6%±5.3%、22.7%±4.6%和28.5%±13.8%,P<0.01)。结论在高脂饮食诱发的雄兔动脉粥样硬化模型中,睾酮可以发挥保护内皮细胞的作用,从而减轻动脉粥样硬化病变的程度。

Aim To investigate the effect of testosterone on endothelial cells in male rabbits atherosclerotic models. Methods Male cholesterol-fed rabbits were divided into four groups at random： placebo group （castrated rabbits treated with placebo）, group with low level testosterone （castrated rabbits treated with testosterone 0.25 mg/kg·d）, group with high level testosterone （castrated rabbits treated with testosterone 2.5 mg/kg·d）, sham operation group. Three months later, the rabbits were sacrificed. Testosterone level, lipids, plasminogen activator inhibitor activity （PAI）, nitric oxide （NO）, endothelin （ET） and angiotensin Ⅱ（Ang Ⅱ） concentration were tested. And the ratio of aortic plaque area to total infima area was examined. Results It showed that testosterone level of placebo group was lower than those of the other groups （2.56 ±0. 28μg/L vs 7.67 ± 0. 61 μg/L, 40.16 ± 0.93 μg/L, 7.79 ± 0.61 μg/L, P〈0.01 ） ; NO content of placebo group was lower than those of the other groups （39.0±9.5 μmol/L vs 62.8±8.9 μmol/L, 56.7±10.3 μmol/L, 51.5±9.3 μmol/L, P 〈0.05 or P 〈0.01）; PAl of placebo group was higher titan that of group with low level testosterone （567.3 ± 53.4 u/L vs 334.4 ± 51.3 u/L, P〈0.01） ; ET of placebo group was higher than that of group with low level testosterone （911.9 ± 15.4 ng/L vs 846.1±43.0 ng/L, P〈0.05） ; and Ang Ⅱof placebo group was higher than that of group with low level testosterone 1687.2 ± 36.5 ng/L vs 624.5 ± 54.5 ng/L, P〈0.050; the ratio of aortic plaque area to total intima area of placebo group was higher than others （46.4% ± 9.3% vs 20.6% ±5.3%, 22.7% ±4.6%, 28.5% ± 13.8%, P〈0.01）. Conclusions Testosterone is a protective factor for endothelial cells, thus to alleviate atherosclerotic lesion for male rabbits fed with high-cholesterol stuff.