氟伐他汀对急性心肌梗死患者循环单核细胞基质金属蛋白酶9和组织因子表达的影响

Effect of Fluvastatin on Expression of Matrix Metalloproteinase-9,Tissue Factor from Circulating Monocytes in Patients with Acute Myocardial Infarction

目的观察氟伐他汀对急性心肌梗死患者循环单核细胞基质金属蛋白酶9、组织因子表达的影响。方法分离30例急性心肌梗死患者和30例正常对照者循环血单核细胞,在体外无血清培养,设两孔,一孔直接培养,一孔加入氟伐他汀(360μg/L)干预48h,酶联免疫吸附法测定上清液中基质金属蛋白酶9、组织因子的含量。结果急性心肌梗死非干预组基质金属蛋白酶9、组织因子均高于正常对照非干预组(351±141μg/L比121±10μg/L,P<0.01;41.4±13.6ng/L比25.5±5.4ng/L,P<0.01)。急性心肌梗死氟伐他汀组较非干预组基质金属蛋白酶9和组织因子表达明显下降(226±89μg/L比351±141μg/L,P<0.01;31.4±11.2ng/L比41.4±13.6ng/L,P<0.05)。结论急性心肌梗死患者循环血单核细胞产生基质金属蛋白酶9、组织因子增加,氟伐他汀能抑制单核细胞表达基质金属蛋白酶9、组织因子。

Aim To investigate the effect of fluvastatin on expression of matrix metalloproteinase-9 （MMP-9）, tissue factor （TF） from the peripheral blood monocytes in patients with acute myocardial infarction （AMI）. Methods Monocytes from 30 AMI patients and 30 control subjects were separated and added into two bores, one cultivated directly, one cultivated with fluvastatin （360 μg/L）. The contents of MMP-9, TF were assayed by enzyme linked immunosorbent assay （ELISA）. Results Compared with control noninterfering group, the productions of MMP-9, TF in AMI noninterfering group were increased significantly （351±141μg/Lvs121±10μg/L, P〈0.01;41.4±13.6ng/Lvs25.5±5.4ng/L, P〈0.01）. In AMI group, compared with the noninterfering group, the productions of MMP-9, TF in fluvastatin group have been decreased significantly （226±89μg/Lvs351±141 μg/L, P〈0.01; 31.4±11.2ng/L vs 41.4±13.6ng/L, P〈0.05）. Conclusion The productions of MMP-9, TF in monocytes from patients with AMI were increased. Fluvastatin inhibited the expression of MMP-9 and TF.