食管癌MMP基因高表达与临床病理特征的关系

Relationship between expression of matrix metalloproteinase and clinicopathological characteristics in esophageal carcinoma

目的:探讨MMP-2,MMP-7,MMP-9基因在食管癌和癌旁组织中的表达以及与食管癌各临床病理指标之间的关系.方法:采用RT-PCR方法分别检测48例食管癌及癌旁组织MMP-2、MMP-7、MMP-9mRNA的阳性表达率及含量.结果:在食管癌组织中MMP-2,MMP-7,MMP-9的阳性表达率分别为81.3%,62.5%和75.0%,明显高于癌旁组织的58.3%,18.8%和52.1%,两两比较差异均有显著性(χ2=5.978,19.045,5.440;P<0.05-0.001).MMP-2,MMP-7,MMP-9表达与肿瘤的大小(r=0.84,0.70,0.48;P<0.05-0.001)、分化高低(r=0.89,0.57,0.91;P<0.05)及临床分期(r=0.81,0.73,0.61;P<0.05)有关,MMP-2mRNA表达量的高低与淋巴结是否转移有关(r=0.048,P<0.05).结论:食管癌组织MMP-2,MMP-7,MMP-9高表达与食管癌的发生、发展及淋巴结转移有关,可作为预测肿瘤转移潜能的指标.

AIM： To study the expression of matrix metalloproteinases （MMP-2, MMP-7, and MMP-9） and their correlations with clinicopathological characteristics in esophageal carcinoma. METHODS： The expression of MMP-2, MMP-7, and MMP-9 mRNA were detected in 48 cases of esophageal carcinoma and cancer-adjacent tissues by the reverse transcriptase polymerase chain reaction （RT-PCR）. RESULTS： The expression of MMP-2, MMP-7, and MMP-9 were significantly higher in esophageal carcinoma tissues than those in cancer-adjacent ones, respectively （X^2= 5.978, 19.045, 5.440; P 〈 0.05-0.001）. The expression of MMP was markedly correlated with the tumor size （r = 0.84, 0.70, 0.48; P 〈 0.05-0.001）, degree of differentiation （r = 0.89, 0.57, 0.91; P 〈 0.05）, and clinical staging （r = 0.81, 0.73, 0.61; P 〈 0.05）. MMP-2 expression was also notably correlated with lymph node metastasis （r=0.048, P 〈 0.05）. CONCLUSION： The over-expression of MMP is correlated with the carcinogenesis, development and metastasis of esophageal carcinoma, which can be used as important indexes to judge the invasion and metastasis of esophageal carcinoma.