趋化因子MIP-1α和MCP-1及其受体CCR-1和CCR-2在慢性髓系白血病细胞中的表达

Expressions of MIP-1α,MCP-1 and Their Receptors CCR-1,CCR-2 in Chronic Myeloid Leukemia Cells

本文旨在研究MIP-1α和MCP-1及其受体CCR-1和CCR-2在慢性髓系白血病细胞中的表达,同时观察P210bcr/abl融合蛋白中酪氨酸激酶对MIP-1α和MCP-1及其受体CCR-1和CCR-2mRNA表达的影响。在bcr/abl融合基因阴性和阳性慢性髓系白血病细胞中,采用半定量RT-PCR方法检测MIP-1α、MCP-1、CCR-1、CCR-2mRNA的表达水平。结果表明MIP-1αmRNA和CCR-1mRNA在bcr/abl融合基因阳性细胞中不表达,但在bcr/abl融合基因阴性细胞中表达,而MCP-1mRNA和CCR-2mRNA在bcr/abl融合基因阳性和阴性细胞中均不表达。当P210bcr/abl融合蛋白中酪氨酸激酶被抑制后,MIP-1α和CCR-1的mRNA表达水平恢复至正常水平。结论:P210bcr/abl融合蛋白抑制慢性髓系白血病细胞中MIP-1α和CCR-1mRNA的表达,但对MCP-1和CCR-2mRNA的表达无影响。

This study was aimed to explore the expression of MIP-1α,MCP-1 and their receptors CCR-1, CCR-2 in ber/abl fusion gene positive CML cells, and to study the effects of P210^bcr/abt fusion protein tyrosine kinase on expression of MIP-lα, MCP-1 and their receptors CCR-1, CCR-2 mRNAs in chronic myeloid leukemia cells. The expression levels of MIP-1α, MCP-1 and their receptors CCR-1, CCR-2 mRNA were detected by semi-quantitative RT-PCR in bcr/abl negative cells, bcr/abl positive cells, and P210^bcr/abl-Rb-C-Box positive cells. The results showed that MIP-lα and CCR-1 mRNAs were expressed in bcr/abl negative cells, but not in positive cells. Both MCP-1 and CCR-2 mRNA cannot be detected in both bcr/abl positive and negative cells. After inhibiting P210^bcr/abl tyrosine kinase activity by Rb-C-Box, expressions of MIP-1α and CCR-1 mRNAs were restored to normal （similar to P210^bcr/abl negative cells）. It is concluded that P210^bcr/abl fusion protein inhibits the expression of MIP-1α and CCR-1 in chronic myeloid leukemia cells, but does not inhibit MCP-1 and CCR-2 mRNA expressions in these leukemia cells.