小鼠急性粒-单核白血病WEHI-3细胞表面免疫应答分子的表达与Fas配体功能研究

Expression of Immune Response Molecules and Function of Fas Ligand on Surface of AML WEHI-3 Cells

为了研究小鼠急性粒-单核白血病细胞株WEHI-3细胞表面免疫应答分子Fas、Fas配体(FasL)、CD80分子表达以及FasL的功能,应用流式细胞术检测WEHI-3细胞表面Fas、FasL和CD80分子表达情况,同时采用氚胸腺嘧啶核苷(3H-TdR)掺入法检测FasL功能。结果表明:WEHI-3细胞表面CD80和Fas表达率分别为(5.06±0.41)%、(6.75±2.31)%(n=5),但FasL表达率高达(63.73±5.23)%(n=5),当WEHI-3(效应细胞,E)和Fas+YAC-1细胞(靶细胞,T)以3∶1、10∶1和30∶1混合培养时,YAC-1细胞的凋亡率分别为(26±4.5)%,(35±3.2)%和(43±2.7)%(n=5)。结论:WEHI-3细胞高表达FasL,低表达Fas和CD80,并能诱导Fas+YAC-1细胞凋亡。

The purpose of this study was to investigate the expression of Fas ,Fas ligand（FasL） and CD80 and function of FasL on the surface of acute myelomonocytic leukemia ceils from WEHI-3 line. The expression of Fas,FasL and CD80 on the surface of WEHI-3 were detected by flow cytometry, the apoptosis of YAC-I cell induced by FasL on the surface of WEHI-3 were detected by 3H-TdR incorporation. The results showed that the expression rate of Fas ,FasL and CD80 on the surface of WEHI-3 cells were （6.75±2.31 ）% （n=5） ,（63.73±5.23）% （n =5） and （5.06±0.41 ）% （n=5）respectively. The apoptosis rate of YAC-1 cells （target cells）co-cultured with WEHI-3 cells （Effector cells） at the rate of 1： 3,1： 10 and 1： 30 were （26±4.5 ） %, （ 35±3.2） % and （43±2.7）% （n=5） respectively. It is concluded that WEHI-3 cells have high expression of FasL and low expression of Fas and CD80 on their cell membrane, and can induce the apoptosis of Fas^＋ YAC-1 cells.