DC与CIK共培养对肝癌细胞杀伤活性的研究

Killing Activity in DC and CIK Co-culture against Hepatocar-cinoma Cells

本研究的目的是观察细胞因子诱导的杀伤细胞(CIK)与同源树突状细胞(DC)共培养后DC-CIK细胞的增殖活性、表型的变化,及其对肝癌细胞细胞毒作用的影响。采集健康供者的外周血单个核细胞(MNC),置于37℃,5%CO2培养箱培养2小时,收集非贴壁细胞用于诱导培养CIK细胞,贴壁细胞诱导分化出成熟DC,将成熟DC和CIK细胞按1∶5的比例混合培养3天,用MTT法检测DC-CIK共培养细胞杀伤SMMC-7721肝癌细胞株的活性。结果显示:DC与CIK细胞共培养后,DC-CIK细胞群的增殖活性和杀伤活性较单纯的CIK细胞更高。结论:DC与CIK共培养细胞是一种增殖活性和细胞毒活性均高于CIK细胞的免疫活性细胞。

This study was aimed to investigate the proliferation activities and phenotype changes of DC, CIK and DCCIK, and their eytotoxieity against hepatoeareinoma cells in co-culture of DC with CIK. Peripheral blood mononuelear cells（PBMNC） were isolated from heathy adult donors. After incubation of PBMNC for 2 hours, DCs were induced from adherent cells by some eytokines and CIKs were generated from non-adherent cells. Mature DCs were harvested after incubation for 9 days, and then were co-cultured with CIK at ratio of 1：5 for 3 days. The eytotoxicity activity against SMMC-7721 hepatoeellular carcinoma cell line was detected by MTT assay. The results showed that CLK cells were able to lyse SMMC-7721 hepatoeellular carcinoma cells at low ratios of effeetor to target. This effect was significantly enhanced by co-culture with DCs. It is concluded that CIK cells have high lytie activity against 7721 hepatocellular carcinoma cell line, which can be enhanced by co-culture with DC. DC-CIK cells are highly effective immune cells.