安宫牛黄注射液对大鼠脑外伤后炎性反应的干预作用

Interventive effect of Angongniuhuang injection on inflammatory response after brain injury in rats

目的观察安宫牛黄注射液对脑外伤后脑内炎性反应因子白细胞介素(IL)-1β、细胞间黏附分子(ICAM)-1、血清S100B蛋白和神经元特异性烯醇化酶(NSE)水平的影响,探讨其对外伤脑组织的保护作用。方法采用Feeney法造成鼠脑挫裂伤模型,在伤后不同时相点用酶联免疫法检测治疗组和对照组脑组织IL-1β、ICAM-1水平、血清S100B蛋白和NSE水平变化。结果除外IL-6β伤后6 h外,伤后6～48 h治疗组脑组织IL-1β、ICAM-1、血清S100B蛋白和NSE均比对照组低(P＜0．05)。结论安宫牛黄注射液能减轻脑外伤后脑组织的炎症反应,对脑组织起一定的保护作用。

Objective To observe the influence of Angongniuhuang injection on the level of interleukin-1 β （IL-1β）, intercellular adhesion molecule-1 （ICAM-1）, serum protein S100B and neuron specific enolase （NSE） after brain injury to explore its protective effect on the injured brain tissues. Methods Brain contusion model was made in rats by Feeney＇s method. Then, the levels of IL-1β and ICAM-1 in the brain tissues and the levels of serum protein S00B and NSE in serum were measured by ELISA method at different time points. Results The level of IL-1β and ICAM-1 in brain tissues and that of S100B protein and NSE in serum in treatment group were lower than that in control group 6-48 hours after injury（P 〈 0.05 ）. Conclusion Angongniuhnang injection can alleviate inflammatory response after brain injury and protect effectively brain tissues.