超声辐射微泡剂抑制SMMC-7721移植瘤的实验研究及其作用机制探讨

Inhibition of SMMC-7721 transplantation tumor after low frequency ultrasound radiate microbubble and mechanism

目的研究低频超声辐射微泡剂对裸鼠移植肿瘤增长抑制情况和组织病理学变化,并探讨其与局部氧自由基代谢产物SOD浓度改变的关系。方法建立SMMC-7721系肝癌Balb/c裸鼠移植瘤模型,随机分为五组,对照组(A),微泡组(B),单纯超声组(C),超声微泡隔日组(D),超声微泡每日组(E)。连续一周观察肿瘤组织病理学损伤,肿瘤体积生长曲线和体积增长率。应用免疫组化的方法分析局部氧自由基代谢产物SOD的变化。分析增长率与SOD浓度之间的相关性。结果超声辐射D组、E组肿瘤体积明显小于对照组(P<0.05),超声辐射D组(73.20%±0.138%),E组(57.24%±0.1653%)肿瘤增长率明显低于对照的A组(121.24%±0.347%)、B组(123.13%±0.232%)和C组(108.74%±0.221%)(P<0.05),病理学检查见瘤内血栓形成和梗死,瘤组织细胞坏死。免疫组化法得出局部SOD阳性率D组(28.83%±2.483%)、E组(32.50%±3.017%)高于A组(11.50%±2.881%),(P<0.01)。并且SOD阳性率与肿瘤生长率间呈负相关(r=-0.663)。结论低频超声辐射微泡剂可以抑制肝癌移植瘤裸小鼠肿瘤的增长,并增加局部氧自由基代谢产物SOD浓度。肿瘤增长率与SOD浓度间有负相关关系。

Objective To investigate the vessel damnification and the restrained growth of SMMC-7721 hepatic cancer planted in athymic mouse after low frequency ultrasound radiate microbubble, and to explore the relation between the tumor growth ratio and the concentration of local free-radicle metabolic product superoxi dismutase （SOD）. Methods SMMC-7721 cells were subcutaneousely planted into Balb/c mice for establishing models. Fifty mice were randomly divided into five groups, control group （A）, simplicity microbubble group （B）, simplicity ultrasound （C）, ultrasound and microbubble qd group （D）, and ultrasound and microbubble qod group （E）. After disposal, histopathology damage was observed. Then, we draw a growth curve and count the ratio of tumor growth. The local concentration of local free-radicle was observed by immunohistochemistry with anti-sod. And the relation between tumor growth ratio and concentration was analyzed. Results Tumor volume of each group has obviously difference. The volume of group D and group E were significantly smaller than those of group A （P〈0.05）. Different ratio of volume growth were statistical difference between group A （121.24% ± 0. 347% ） and group D （73.20% ±0. 138% ） and E （57.24% ±0. 1653% ） （P〈0.01）. Expression of SOD in tumor tissue of group D （28.83 % ± 2. 483 % ） and E （32. 50 % ± 3. 017 % ） were significantly higher than those in group A （ 11. 50 % ± 2. 881%） （P〈0. 01）, and had negative correlation with tumor growth ratio （r=-0. 663）. Conclusion Low-frequency ultrasound radiate with microbubble can inhibit growth of the tumor which planted in mice, increase the concentration of local free-radicle metabolic product SOD. And tumor growth ratio negatively relates to the local free-radicle metabolic product SOD.